Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,793,867	C→A	A378D (GCT→GAT)	gabT →	4‑aminobutyrate aminotransferase GabT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,793,867	0	C	A	100.0%	71.7 / NA	20	A378D (GCT→GAT)	gabT	4‑aminobutyrate aminotransferase GabT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/11);  total (9/11)

GGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTAT  >  NC_000913/2793821‑2793913                                               |                                               ggCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATcg                                             >  1:1270220/1‑50 (MQ=255)  gCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATcgc                                            >  1:2237245/1‑50 (MQ=255)   cGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATcgcg                                           <  1:546137/50‑1 (MQ=255)    gATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATCgcgc                                          >  1:841119/1‑50 (MQ=255)      tCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATCGCGccc                                        <  1:483137/50‑1 (MQ=255)        acaacaAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATCGCGCCcg                                       <  1:790694/49‑1 (MQ=255)         caacaaGCCGGACGCCAAACTCACCGCCGAGATCGTGGATCGCGCCcgc                                      >  1:1044685/1‑49 (MQ=255)              aGCCGGACGCCAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATaaa                                <  1:1472905/50‑1 (MQ=255)                    aCGCCAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTg                          <  1:2275426/50‑1 (MQ=255)                    aCGCCAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTg                          <  1:278320/50‑1 (MQ=255)                       ccAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGAtt                       >  1:2161875/1‑50 (MQ=255)                        cAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGAttc                      >  1:1851314/1‑50 (MQ=255)                              cACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTtctct                >  1:1498397/1‑50 (MQ=255)                               aCCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTtctc                 <  1:2313670/48‑1 (MQ=255)                               aCCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTtctc                 <  1:1008321/48‑1 (MQ=255)                                    cgAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCg          >  1:1930072/1‑50 (MQ=255)                                     gagaTCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCgg         >  1:1324641/1‑50 (MQ=255)                                      agaTCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGc        <  1:2223544/50‑1 (MQ=255)                                           gTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGt    <  1:711554/49‑1 (MQ=255)                                            tGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTAt  <  1:2175540/50‑1 (MQ=255)                                               |                                               GGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTAT  >  NC_000913/2793821‑2793913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑