Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,813,364	T→C	V250A (GTC→GCC)	emrB →	multidrug efflux pump membrane subunit EmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,813,364	0	T	C	100.0%	79.9 / NA	22	V250A (GTC→GCC)	emrB	multidrug efflux pump membrane subunit EmrB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/11);  total (11/11)

TCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTT  >  NC_000913/2813319‑2813406                                              |                                           tcatcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTg                                         <  1:829719/49‑1 (MQ=255)  catcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTgg                                        >  1:270515/1‑49 (MQ=255)    tcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAg                                     <  1:1407035/50‑1 (MQ=255)      cTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCt                                   <  1:2002872/50‑1 (MQ=255)      cTTACCGTGGTGGCGGTGGTGGCTATCTGATTCCTGATTGCCTGGGAGc                                    <  1:2183680/49‑1 (MQ=38)        tACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGa                                 >  1:195969/1‑50 (MQ=255)         aCCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGAc                                >  1:556484/1‑50 (MQ=255)            gtggtgGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACcga                             >  1:2209339/1‑50 (MQ=255)                    ggtggtGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAcc                     >  1:1260398/1‑50 (MQ=255)                    ggtggtGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAcc                     >  1:1929453/1‑50 (MQ=255)                     gtggtgGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAccc                    >  1:521786/1‑50 (MQ=255)                     gtggtgGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAccc                    >  1:640062/1‑50 (MQ=255)                      tggtggCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCg                   <  1:1924067/50‑1 (MQ=255)                          ggCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAg               >  1:191088/1‑50 (MQ=255)                           gCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAg               <  1:2187026/49‑1 (MQ=255)                            cTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTc             <  1:1683212/50‑1 (MQ=255)                            cTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTc             <  1:54256/50‑1 (MQ=255)                                 tGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCt        >  1:2253313/1‑50 (MQ=255)                                  gCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTg       >  1:1796660/1‑50 (MQ=255)                                   cTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGAt          <  1:224174/46‑1 (MQ=255)                                   cTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCt        <  1:554843/48‑1 (MQ=255)                                       cTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCgtt  <  1:2165545/50‑1 (MQ=255)                                              |                                           TCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTT  >  NC_000913/2813319‑2813406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑