Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,843,412	C→T	R12H (CGT→CAT)	hycH ←	formate hydrogenlyase assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,843,412	0	C	T	100.0%	61.2 / NA	18	R12H (CGT→CAT)	hycH	formate hydrogenlyase assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/6);  total (12/6)

CCTGCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGC  >  NC_000913/2843365‑2843455                                                |                                            ccTGCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATgg                                           >  1:1888508/1‑50 (MQ=255)   tgctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt                                         >  1:23280/1‑50 (MQ=255)    gctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt                                         >  1:789426/1‑49 (MQ=255)     ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTc                                        <  1:976044/49‑1 (MQ=255)              ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGa                              <  1:298610/50‑1 (MQ=255)                  ggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAacac                          >  1:1545473/1‑50 (MQ=255)                    cgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAAcacca                        >  1:767592/1‑50 (MQ=255)                     gTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAAcaccac                       <  1:846797/50‑1 (MQ=255)                      tGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACaccacc                      >  1:46548/1‑50 (MQ=255)                            cGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCa                >  1:1128596/1‑50 (MQ=255)                              ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt              >  1:2021609/1‑50 (MQ=255)                              ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt              >  1:109463/1‑50 (MQ=255)                                   atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCg         >  1:2097038/1‑50 (MQ=255)                                    tcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCgg        <  1:518820/50‑1 (MQ=255)                                      aaTAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGa       <  1:2161023/49‑1 (MQ=255)                                      aaTAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGa       >  1:987477/1‑49 (MQ=255)                                       aTAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATa     <  1:1794972/50‑1 (MQ=255)                                          aaTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcgc  >  1:1408633/1‑50 (MQ=255)                                                |                                            CCTGCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGC  >  NC_000913/2843365‑2843455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑