Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,090,282	C→T	intergenic (+30/‑65)	yggI → / → endA	conserved protein YggI/DNA‑specific endonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,090,282	0	C	T	100.0%	88.0 / NA	26	intergenic (+30/‑65)	yggI/endA	conserved protein YggI/DNA‑specific endonuclease I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/17);  total (9/17)

AGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTC  >  NC_000913/3090236‑3090326                                               |                                             aGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTgg                                           >  1:756150/1‑50 (MQ=255) aGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTgg                                           >  1:1337076/1‑50 (MQ=255)    tGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCt                                         >  1:2289281/1‑49 (MQ=255)      gTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTg                                        <  1:1268713/48‑1 (MQ=255)      gTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTg                                        <  1:1995908/48‑1 (MQ=255)      gTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGAt                                      >  1:1034789/1‑50 (MQ=255)       ttGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGAtt                                     <  1:289504/50‑1 (MQ=255)          cGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGc                                   <  1:2335622/49‑1 (MQ=255)              aTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAcc                              <  1:2136435/50‑1 (MQ=255)               tAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCa                             <  1:1052183/50‑1 (MQ=255)                aaCCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCa                             >  1:599853/1‑49 (MQ=255)                    aTCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                          >  1:1451893/1‑48 (MQ=255)                    aTCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                          >  1:923986/1‑48 (MQ=255)                      cTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGc                      >  1:1457040/1‑50 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCttt                   <  1:705199/50‑1 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCt                     <  1:1785680/48‑1 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCt                     <  1:1376864/48‑1 (MQ=255)                          aCTATCAGGAACTTTCCTGATTTGGCTGATTGTATACCAAAACAGCTTTc                  <  1:1435659/50‑1 (MQ=255)                          aCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTc                  >  1:1922331/1‑50 (MQ=255)                           cTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCg                 <  1:1129570/50‑1 (MQ=255)                                aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACg            <  1:314416/50‑1 (MQ=255)                                  gAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGtt          <  1:829576/50‑1 (MQ=255)                                    aCTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGc        <  1:624841/50‑1 (MQ=255)                                     cTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCt       <  1:1120832/50‑1 (MQ=255)                                        tCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTg      <  1:989986/48‑1 (MQ=255)                                          cTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTc  <  1:1182019/50‑1 (MQ=255)                                               |                                             AGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTC  >  NC_000913/3090236‑3090326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑