Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	257,794	T→C	intergenic (+23/‑35)	frsA → / → crl	fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	257,794	0	T	C	100.0%	122.7 / NA	32	intergenic (+23/‑35)	frsA/crl	fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/11);  total (21/11)

CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACC  >  NC_000913/257747‑257839                                                |                                              cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                             <  1:924949/50‑1 (MQ=255)  tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                            >  1:822561/1‑50 (MQ=255)    gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                            <  1:1252202/48‑1 (MQ=255)       cGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                       >  1:1749663/1‑50 (MQ=255)       cGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                       >  1:1464486/1‑50 (MQ=255)           aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                   <  1:1065401/50‑1 (MQ=255)              cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGc                                <  1:1635944/50‑1 (MQ=255)                cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                              >  1:982904/1‑50 (MQ=255)                   gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                            >  1:971179/1‑49 (MQ=255)                   gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATc                             <  1:1552562/48‑1 (MQ=255)                    tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac                           >  1:2184158/1‑49 (MQ=255)                      ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                         >  1:2326438/1‑49 (MQ=255)                        aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                      >  1:1356877/1‑50 (MQ=255)                         aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                      >  1:593531/1‑49 (MQ=255)                         aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                      <  1:2097512/49‑1 (MQ=255)                          aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                      >  1:1115839/1‑48 (MQ=255)                          aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                      >  1:448948/1‑48 (MQ=255)                          aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGga                    <  1:1134041/50‑1 (MQ=255)                             tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                  >  1:1358398/1‑49 (MQ=255)                              ttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                  >  1:1609240/1‑48 (MQ=255)                                 cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc              >  1:1953361/1‑49 (MQ=255)                                   aaaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt           >  1:2321464/1‑50 (MQ=255)                                    aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt           <  1:620318/49‑1 (MQ=255)                                    aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATg          <  1:2306862/50‑1 (MQ=255)                                     aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa         >  1:930690/1‑50 (MQ=255)                                        ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt      >  1:382239/1‑50 (MQ=255)                                        ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt      >  1:1314063/1‑50 (MQ=255)                                         tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt      >  1:2281357/1‑49 (MQ=255)                                           ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa    <  1:914377/49‑1 (MQ=255)                                           ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc   >  1:25622/1‑50 (MQ=255)                                            cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc  <  1:2199115/50‑1 (MQ=255)                                             aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc  >  1:967948/1‑49 (MQ=255)                                                |                                              CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACC  >  NC_000913/257747‑257839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑