Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,093,157	T→C	L74P (CTC→CCC)	yqgE →	DUF179 domain‑containing protein YqgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,093,157	0	T	C	100.0%	83.1 / NA	23	L74P (CTC→CCC)	yqgE	DUF179 domain‑containing protein YqgE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/15);  total (8/15)

CGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCG  >  NC_000913/3093112‑3093203                                              |                                               cGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCCcggc                                            >  1:2164994/1‑50 (MQ=255)    aGCCGCGTGATGAATCAGTCCGTCTGGATAAACCGGTTATGCCCGGCGGt                                         <  1:1265677/50‑1 (MQ=255)      ccGCGTGATGAATCAATCCGTCTGGATAATCCGGTTATGCCCGGCGGTcc                                       <  1:1494259/50‑1 (MQ=255)       cgcgTGATGAATCAATCCGTCTGGATAAACCGGTTATGCCCGGCGGTCCg                                      >  1:1705775/1‑50 (MQ=255)         cgTGATGAATCAATCCGTCTGGATAAACCGGTTATGCCCGGCGGTCCgct                                    >  1:431925/1‑50 (MQ=255)          gTGATGAATCAATCCGTCTGGATAAACCGGTTATGCCCGGCGGTCCgctg                                   <  1:2276934/50‑1 (MQ=255)            gatgaATCAATCCGTCTGGATAAACCGGTTATGCCCGGCGGTCCgctggc                                 >  1:2285800/1‑50 (MQ=255)              tgaATCAATCCGTCTGGATAAACCGGTTATGCCCGGCGGTCCgctggctg                               <  1:1971217/50‑1 (MQ=255)                    aatcCGTCTGGATAAACCGGTTATGCCCGGCGGTCCGCTGGCTGAAGAt                          <  1:14239/49‑1 (MQ=255)                    aatcCGTCTGGATAAACCGGTTATGCCCGGCGGTCCGCTGGCTGAAGAt                          <  1:1972397/49‑1 (MQ=255)                     atcCGTCTGGATAAACCGGTTATGCCCGGCGGTCCGCTGGCTGAAGATcg                        <  1:596707/50‑1 (MQ=255)                     atcCGTCTGGATAAACCGGTTATGCCCGGCGGTCCGCTGGCTGAAGATcg                        >  1:702088/1‑50 (MQ=255)                      tcCGTCTGGATAAACCGGTTATGCCCGGCGGTCCGCTGGCTGAAGATcgc                       <  1:786620/50‑1 (MQ=255)                         gTCTGGATAAACCGGTTATGCCCGGCGGTCCGCTGGCTGAAGATCGCggg                    >  1:1685803/1‑50 (MQ=255)                          tCTGGATAAACCGGTTACGCCCGGCGGTCCGCTGGCTGAAGATCGCGGGt                   <  1:1979554/50‑1 (MQ=255)                                tAAACCGGTTATGCCCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTAttt             <  1:336939/50‑1 (MQ=255)                                     cGGTTATGCCCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCAt        <  1:531989/50‑1 (MQ=255)                                      ggTTATGCCCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATa       >  1:2057357/1‑50 (MQ=255)                                       gTTATGCCCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATAc      <  1:1117970/50‑1 (MQ=255)                                       gTTATGCCCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATAc      >  1:908361/1‑50 (MQ=255)                                        ttATGCCCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACt     <  1:262833/50‑1 (MQ=255)                                        ttATGCCCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACt     <  1:2127095/50‑1 (MQ=255)                                           tGCCCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCg  <  1:2152543/50‑1 (MQ=255)                                              |                                               CGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCG  >  NC_000913/3093112‑3093203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑