Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,354,900	T→A	L59* (TTA→TAA)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,354,900	0	T	A	100.0%	34.4 / NA	11	L59* (TTA→TAA)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (5/6);  total (5/6)

GATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGG  >  NC_000913/3354856‑3354949                                             |                                                  gATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTAACaaa                                              <  1:1314350/50‑1 (MQ=255)        gCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTAACAAAAACCGGa                                       >  1:1204894/1‑50 (MQ=255)           gATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTAACAAAAACCGGATCg                                    <  1:1422806/50‑1 (MQ=255)                   aaCCGGCGACGGTTGCGGCTTGCTGTAACAAAAACCGGATCGCTTTTTTc                            <  1:955324/50‑1 (MQ=255)                    aCCGGCGACGGTTGCGGCTTGCTGTAACAAAAACCGGATCGCTTTTTTCg                           <  1:1935824/50‑1 (MQ=255)                     ccGGCGACGGTTGCGGCTTGCTGTAACAAAAACCGGATCGCTTTTTTCGc                          <  1:1004816/50‑1 (MQ=255)                                  cGGCTTGCTGTAACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAg              >  1:471079/1‑49 (MQ=255)                                   ggCTTGCTGTAACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAg              >  1:837462/1‑48 (MQ=255)                                     cTTGCTGTAACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAgc          <  1:837782/50‑1 (MQ=255)                                       tGCTGTAACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAgcgc        >  1:1661456/1‑50 (MQ=255)                                          tGTAACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGc     >  1:2054/1‑50 (MQ=255)                                             tACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTgg  >  1:1102609/1‑50 (MQ=255)                                             |                                                  GATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGG  >  NC_000913/3354856‑3354949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑