Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,150	C→T	K90K (AAG→AAA)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,150	0	C	T	100.0%	131.7 / NA	37	K90K (AAG→AAA)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/26);  total (11/26)

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTT  >  NC_000913/3477104‑3477194                                               |                                             cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                            <  1:937023/49‑1 (MQ=255)   aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                         <  1:1932072/50‑1 (MQ=255)    aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                        <  1:1879651/50‑1 (MQ=255)     tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                       <  1:2063338/50‑1 (MQ=255)       cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                      >  1:1719039/1‑49 (MQ=255)       cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                     <  1:612245/50‑1 (MQ=255)       cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                     <  1:2279450/50‑1 (MQ=255)        ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                     >  1:1214154/1‑49 (MQ=255)               gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                             >  1:347281/1‑50 (MQ=255)                tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                            <  1:1986949/50‑1 (MQ=255)                tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                            >  1:1390183/1‑50 (MQ=255)                   gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                          >  1:371181/1‑49 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                           <  1:1720709/47‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                          <  1:1102950/48‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                        >  1:1215200/1‑50 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa                         <  1:1583992/49‑1 (MQ=255)                      cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                       <  1:1264944/49‑1 (MQ=255)                        aTCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                     >  1:1500056/1‑49 (MQ=255)                         tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                     >  1:95396/1‑48 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                     <  1:570553/44‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                   <  1:891622/46‑1 (MQ=255)                             ttGGTCGAAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                 <  1:1009416/48‑1 (MQ=39)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCg                <  1:2283487/47‑1 (MQ=255)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt              >  1:395030/1‑49 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc             <  1:1073802/49‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc             <  1:966006/49‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc             <  1:475105/49‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa            <  1:2034898/50‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa            <  1:1837563/50‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa            <  1:1658778/50‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa            <  1:377122/50‑1 (MQ=255)                                   ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt         >  1:1982974/1‑50 (MQ=255)                                   ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt         >  1:1910128/1‑50 (MQ=255)                                    gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg        <  1:2065905/50‑1 (MQ=255)                                      gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt      <  1:189023/50‑1 (MQ=255)                                       agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc     <  1:665858/50‑1 (MQ=255)                                        gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt    <  1:1367008/50‑1 (MQ=255)                                           tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt  <  1:1044878/49‑1 (MQ=255)                                               |                                             CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTT  >  NC_000913/3477104‑3477194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑