Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,527,760	A→G	N431S (AAC→AGC)	igaA →	inner membrane protein ‑ inhibits the Rcs signaling pathway

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,527,760	0	A	G	100.0%	54.6 / NA	15	N431S (AAC→AGC)	igaA	inner membrane protein ‑ inhibits the Rcs signaling pathway
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/4);  total (11/4)

AACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGT  >  NC_000913/3527713‑3527799                                                |                                        aaCCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcg                                       <  1:979338/50‑1 (MQ=255)  aCCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcga                                      >  1:2122563/1‑50 (MQ=255)  aCCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcga                                      <  1:2138925/50‑1 (MQ=255)   ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac                                     <  1:1843050/50‑1 (MQ=255)     aaTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGc                                   >  1:519917/1‑50 (MQ=255)            cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa                            >  1:729096/1‑50 (MQ=255)               ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTg                         >  1:220024/1‑50 (MQ=255)                     cGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTa                   >  1:932983/1‑50 (MQ=255)                      gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc                  >  1:282741/1‑50 (MQ=255)                       tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc                  >  1:488137/1‑49 (MQ=255)                           aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGa              <  1:1906494/49‑1 (MQ=255)                            cTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAAt            <  1:714177/50‑1 (MQ=255)                               cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATc           <  1:1165825/48‑1 (MQ=255)                                 cGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGaa       >  1:294742/1‑50 (MQ=255)                                  gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc      >  1:603650/1‑50 (MQ=255)                                   cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt     >  1:751065/1‑50 (MQ=255)                                    aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg    >  1:756449/1‑50 (MQ=255)                                      atcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt  >  1:418324/1‑50 (MQ=255)                                      atcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt  <  1:1806555/50‑1 (MQ=255)                                                |                                        AACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGT  >  NC_000913/3527713‑3527799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑