Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	86.5 / NA	19	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (10/9);  total (10/9)

CCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACAC  >  NC_000913/765141‑765233                                                 |                                             ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                              >  1:1802842/1‑49 (MQ=255)  cTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                              >  1:1497103/1‑48 (MQ=255)    tttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                            >  1:1840027/1‑47 (MQ=255)     ttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAttat                                           <  1:2240075/48‑3 (MQ=255)     ttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                            >  1:210429/1‑46 (MQ=255)          tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                    <  1:1546813/49‑1 (MQ=38)          tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                    >  1:1614602/1‑49 (MQ=38)                tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                              <  1:287456/49‑1 (MQ=38)                  gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAtt                             <  1:887701/48‑1 (MQ=38)                  gCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                            >  1:2329066‑M1/1‑49 (MQ=255)                    gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                            >  1:66155‑M1/1‑47 (MQ=255)                        atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                      >  1:2337963‑M1/1‑43 (MQ=255)                         ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                     <  1:1045305‑M1/49‑8 (MQ=255)                         ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                     <  1:2164913‑M1/49‑8 (MQ=255)                             cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                 >  1:610635‑M1/1‑38 (MQ=255)                              gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                 <  1:2188063‑M1/48‑12 (MQ=255)                                  cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaa            <  1:49090‑M1/49‑17 (MQ=255)                                  cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccga             <  1:440587‑M1/48‑16 (MQ=255)                                     tATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc          >  1:1445129‑M1/1‑30 (MQ=255)                                     tATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc          >  1:2177732‑M1/1‑30 (MQ=255)                                      aTCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca        >  1:487881‑M1/1‑29 (MQ=255)                                       tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcc         <  1:2077195‑M1/47‑20 (MQ=255)                                        cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca      >  1:903631‑M1/1‑27 (MQ=255)                                            gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg  >  1:240378‑M1/1‑23 (MQ=255)                                                 |                                             CCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACAC  >  NC_000913/765141‑765233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑