Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	972,866	Δ1 bp	coding (536/780 nt)	elyC ←	envelope biogenesis factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	972,863	0	G	.	100.0%	55.4 / NA	14	coding (539/780 nt)	elyC	envelope biogenesis factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (5/9);  total (5/9)

AGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTT  >  NC_000913/972816‑972905                                                |                                           aGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAgg                                           <  1:852679/49‑1 (MQ=255)  gATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAggg                                          >  1:831159/1‑49 (MQ=255)            cgcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAAt                               >  1:2326791/1‑49 (MQ=39)             gcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATc                              <  1:386379/49‑1 (MQ=39)                 cAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCt                          <  1:1241530/49‑1 (MQ=39)                 cAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCt                          >  1:2084393/1‑49 (MQ=39)                 cAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCt                          <  1:2189726/49‑1 (MQ=39)                  aGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTg                         <  1:1380235/49‑1 (MQ=39)                    aTGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGtt                       <  1:1921196/49‑1 (MQ=39)                         aTGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCAct                  <  1:502293/49‑1 (MQ=39)                            cGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCActgct               <  1:70291/49‑1 (MQ=39)                             ggAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCActgctg              >  1:291075/1‑49 (MQ=39)                                 gTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGc          >  1:1655567/1‑49 (MQ=39)                                      cagcagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttctt     <  1:1636990/49‑1 (MQ=39)                                       agcagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttcttc    >  1:2086229/1‑49 (MQ=39)                                         cagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttcttctt  <  1:2040705/49‑1 (MQ=39)                                                |                                           AGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTT  >  NC_000913/972816‑972905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑