Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,720,597	Δ1 bp	coding (4/153 nt)	hokA ←	small toxic polypeptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,720,596	0	G	.	100.0%	96.0 / NA	21	coding (5/153 nt)	hokA	small toxic polypeptide
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (18/3);  total (18/3)

CGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTA  >  NC_000913/3720553‑3720636                                            |                                         cGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTc                                    >  1:2074281/1‑49 (MQ=39) cGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTc                                    >  1:2071564/1‑49 (MQ=39) cGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTc                                    >  1:1888791/1‑49 (MQ=39)  gTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTcc                                   >  1:1708553/1‑49 (MQ=39)       aCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcc                              >  1:1148187/1‑49 (MQ=255)        cAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcct                             >  1:985060/1‑49 (MQ=255)            tCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtg                         <  1:1493075/49‑1 (MQ=255)               cTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTgtgt                      >  1:1943059/1‑49 (MQ=255)               cTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTgtgt                      >  1:2295711/1‑49 (MQ=255)                 aTTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt                     >  1:410535/1‑48 (MQ=255)                 aTTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt                     >  1:627982/1‑48 (MQ=255)                  ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGa                   <  1:568389/49‑1 (MQ=255)                   tAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAt                  >  1:273544/1‑49 (MQ=255)                   tAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAt                  >  1:317207/1‑49 (MQ=255)                    aaagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt                 >  1:1624389/1‑49 (MQ=255)                    aaagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt                 >  1:208755/1‑49 (MQ=255)                     aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt                 <  1:110185/48‑1 (MQ=255)                        aaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc              >  1:1414623/1‑48 (MQ=255)                        aaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc              >  1:656223/1‑48 (MQ=255)                              aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAg        >  1:683688/1‑48 (MQ=255)                                   tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaac  >  1:61513‑M1/1‑46 (MQ=255)                                            |                                         CGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTA  >  NC_000913/3720553‑3720636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑