Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,847,724	(C)6→7	coding (285/1044 nt)	selD ←	selenide, water dikinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,847,718	1	.	C	100.0%	56.7 / NA	16	G97G (GGC→GGG)	selD	selenide, water dikinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (11/5);  total (11/5)

TGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑CCCCCCATCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCA  >  NC_000913/1847675‑1847760                                             |                                           tGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑ccccc                                       >  1:1263622/1‑49 (MQ=255)   ttAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑cccccc                                      <  1:1419145/48‑1 (MQ=255)    tAATCGGCCTGCCGAGGATCGCAATCGCCATAATCGGTTTG‑ccccccca                                    >  1:1192715/1‑47 (MQ=38)    tAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑ccccccca                                    >  1:329956/1‑47 (MQ=255)         ggCCAGCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCCATcgc                                 >  1:1007865/1‑48 (MQ=38)           ccAGCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCCATcgcg                                >  1:158666/1‑47 (MQ=38)             aGCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCCATcgcg                                >  1:404222/1‑45 (MQ=38)                cGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGatat                         <  1:629133/49‑1 (MQ=38)                cGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGatat                         <  1:294298/49‑1 (MQ=38)                    gATCGCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCt                     >  1:2334455/1‑49 (MQ=38)                     aTCGCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCt                     >  1:1567532/1‑48 (MQ=38)                         cAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATgg                >  1:652234/1‑49 (MQ=38)                          aaTCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATGGc               >  1:1665225/1‑49 (MQ=39)                          aaTCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATGGc               >  1:2121672/1‑49 (MQ=39)                           aTCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATGGCg              >  1:1928971/1‑49 (MQ=255)                           aTCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATGGCg              <  1:430447/49‑1 (MQ=255)                              gCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATGGCGt             >  1:1975459/1‑47 (MQ=255)                                      cGGTTTGCCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGTcgccg    >  1:100916/1‑48 (MQ=38)                                       ggTTTGCCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCa  <  1:475762/49‑1 (MQ=38)                                       ggTTTGCCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCa  <  1:1276593/49‑1 (MQ=38)                                             |                                           TGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑CCCCCCATCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCA  >  NC_000913/1847675‑1847760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑