Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,086	C→T	intergenic (‑300/‑77)	yaaI ← / → dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,086	0	C	T	100.0%	93.2 / NA	26	intergenic (‑300/‑77)	yaaI/dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/9);  total (17/9)

GTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACC  >  NC_000913/12042‑12128                                             |                                           gtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGtt                                        <  1:262765/49‑1 (MQ=255) gtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGtt                                        >  1:2162603/1‑49 (MQ=255) gtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGt                                         <  1:2341477/48‑1 (MQ=255)  tagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTg                                       <  1:518477/49‑1 (MQ=255)        aCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                   >  1:927025/1‑47 (MQ=255)        aCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCa                                 <  1:983794/49‑1 (MQ=255)         ccGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                   >  1:939096/1‑46 (MQ=255)           gCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGAc                              >  1:2356716/1‑49 (MQ=255)            cAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACg                             >  1:1624998/1‑49 (MQ=255)             agtgagtgAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGt                            >  1:541074/1‑49 (MQ=255)                 agtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCg                        >  1:161605/1‑49 (MQ=255)                 agtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCg                        <  1:276898/49‑1 (MQ=255)                    gagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGccc                     >  1:1655419/1‑49 (MQ=255)                    gagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGccc                     >  1:2052020/1‑49 (MQ=255)                      gtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGcccc                    >  1:2309257/1‑48 (MQ=255)                       tCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTa                  >  1:22222/1‑49 (MQ=255)                           cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa               <  1:1232925/48‑1 (MQ=255)                           cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa               >  1:491576/1‑48 (MQ=255)                             aaaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa               >  1:679530/1‑46 (MQ=255)                               aaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGAc          >  1:507375/1‑49 (MQ=255)                               aaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGAc          >  1:235604/1‑49 (MQ=255)                                aaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACt         <  1:2315165/49‑1 (MQ=255)                                   tGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTca       >  1:993022/1‑48 (MQ=255)                                       aTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAAcc  <  1:2158550/49‑1 (MQ=255)                                       aTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAAcc  <  1:139887/49‑1 (MQ=255)                                       aTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAAc   >  1:1356829/1‑48 (MQ=255)                                             |                                           GTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACC  >  NC_000913/12042‑12128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑