Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,979,769	C→T	A390V (GCG→GTG)	wzyE →	enterobacterial common antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,979,769	0	C	T	100.0%	230.6 / NA	58	A390V (GCG→GTG)	wzyE	enterobacterial common antigen polymerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/29);  total (29/29)

CTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGT  >  NC_000913/3979724‑3979813                                              |                                             cTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGAt                                           <  1:1178256/49‑1 (MQ=255) cTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGAt                                           <  1:536478/49‑1 (MQ=255) cTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGAt                                           >  1:466136/1‑49 (MQ=255) cTAACCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGAt                                           <  1:330724/49‑1 (MQ=39)  tAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATc                                          >  1:2328901/1‑49 (MQ=255)  tAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATc                                          >  1:532522/1‑49 (MQ=255)    aTCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATc                                          <  1:589632/47‑1 (MQ=255)    aTCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATc                                          <  1:2053292/47‑1 (MQ=255)     tCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTc                                       <  1:747876/49‑1 (MQ=255)     tCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTc                                       <  1:717613/49‑1 (MQ=255)      cGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTc                                       >  1:155923/1‑48 (MQ=255)       gCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCaa                                     >  1:402087/1‑49 (MQ=255)        cTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAat                                    <  1:1536984/49‑1 (MQ=255)         tataAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAata                                   >  1:429859/1‑49 (MQ=255)         tataAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAata                                   >  1:1725707/1‑49 (MQ=255)          ataAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAatat                                  >  1:1663907/1‑49 (MQ=255)          ataAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAatat                                  >  1:1779645/1‑49 (MQ=255)           taAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAatat                                  >  1:1707114/1‑48 (MQ=255)           taAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAatat                                  >  1:2097595/1‑48 (MQ=255)           taAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAatat                                  <  1:1048123/48‑1 (MQ=255)            aaaGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGa                                >  1:1025290/1‑49 (MQ=255)             aaGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGa                                <  1:165521/48‑1 (MQ=255)             aaGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGa                                <  1:631170/48‑1 (MQ=255)             aaGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGAt                               <  1:1998650/49‑1 (MQ=255)               gCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCg                             <  1:932539/49‑1 (MQ=255)                cTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGt                            <  1:1050693/49‑1 (MQ=255)                cTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGt                            >  1:587981/1‑49 (MQ=255)                cTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGt                            <  1:2111567/49‑1 (MQ=255)                cTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGt                            >  1:23328/1‑49 (MQ=255)                cTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGt                            <  1:1586929/49‑1 (MQ=255)                cTGCGATACTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGt                            <  1:367290/49‑1 (MQ=255)                 tGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTg                           >  1:1986852/1‑49 (MQ=255)                   cGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTg                           <  1:24850/47‑1 (MQ=255)                    gATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTg                        <  1:551913/49‑1 (MQ=255)                     atatTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTgg                       <  1:318295/49‑1 (MQ=255)                     atatTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTgg                       <  1:847994/49‑1 (MQ=255)                      tatTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGgc                      <  1:76758/49‑1 (MQ=255)                       atTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGgcg                     >  1:2122664/1‑49 (MQ=255)                       atTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGCGCTGgcg                     >  1:1622562/1‑49 (MQ=39)                        ttGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGgcgc                    >  1:200423/1‑49 (MQ=255)                        ttGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGgcg                     <  1:503711/48‑1 (MQ=255)                           cacaGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGt                  >  1:67236/1‑48 (MQ=255)                            acaGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTg                 >  1:611991/1‑48 (MQ=255)                              aGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAg              <  1:255098/49‑1 (MQ=255)                                tttCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAggg            <  1:911939/49‑1 (MQ=255)                                tttCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAggg            >  1:47152/1‑49 (MQ=255)                                tttCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAggg            >  1:2323087/1‑49 (MQ=255)                                  tCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCt          >  1:300335/1‑49 (MQ=255)                                   cTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCt          <  1:395192/48‑1 (MQ=255)                                      cTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGAt      >  1:2159246/1‑49 (MQ=255)                                       tttGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGAtt     >  1:1058174/1‑49 (MQ=255)                                         tGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGAtt     <  1:701267/47‑1 (MQ=255)                                         tGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTc    <  1:1670877/48‑1 (MQ=255)                                         tGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCg   >  1:2098353/1‑49 (MQ=255)                                         tGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCg   >  1:2107026/1‑49 (MQ=255)                                         tGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCg   >  1:1391745/1‑49 (MQ=255)                                          ggggTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGt  >  1:1652853/1‑49 (MQ=255)                                           gggTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGt  <  1:258942/48‑1 (MQ=255)                                              |                                             CTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGT  >  NC_000913/3979724‑3979813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑