Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,124	T→C	W140R (TGG→CGG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,124	0	T	C	100.0%	112.8 / NA	30	W140R (TGG→CGG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/13);  total (17/13)

AAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAT  >  NC_000913/4438079‑4438167                                              |                                            aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                           >  1:1170017/1‑48 (MQ=255)   aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                        <  1:107241/49‑1 (MQ=255)    aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                       <  1:2073486/49‑1 (MQ=255)        gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                    >  1:1421524/1‑48 (MQ=255)        gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                   <  1:943662/49‑1 (MQ=255)         tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                  >  1:1664290/1‑49 (MQ=255)           aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTAc                                 <  1:1141512/48‑1 (MQ=255)             tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                               >  1:1842599/1‑48 (MQ=255)             tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                               >  1:1817352/1‑48 (MQ=255)               tCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                             >  1:1551326/1‑48 (MQ=255)                 cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                          <  1:21873/49‑1 (MQ=255)                   ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                         >  1:929054/1‑48 (MQ=255)                        aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                   >  1:63639/1‑49 (MQ=255)                        aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                   >  1:60276/1‑49 (MQ=255)                          aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                 >  1:1298071/1‑49 (MQ=255)                           aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                 >  1:421146/1‑48 (MQ=255)                            aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa               >  1:1521797/1‑49 (MQ=255)                             aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt              <  1:433394/49‑1 (MQ=255)                             aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt              <  1:832269/49‑1 (MQ=255)                             aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt              <  1:1543056/49‑1 (MQ=255)                                gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc           <  1:2154161/49‑1 (MQ=255)                                gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc           <  1:1472810/49‑1 (MQ=255)                                 aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg          <  1:806174/49‑1 (MQ=255)                                  cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg          >  1:1462492/1‑48 (MQ=255)                                  cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg          >  1:938986/1‑48 (MQ=255)                                   gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa        <  1:554593/49‑1 (MQ=255)                                     ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa        >  1:1179306/1‑47 (MQ=255)                                      tGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg     >  1:758329/1‑49 (MQ=255)                                        tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa   >  1:2280454/1‑49 (MQ=255)                                          tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt  <  1:1259899/48‑1 (MQ=255)                                              |                                            AAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAT  >  NC_000913/4438079‑4438167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑