Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	70.8 / NA	19	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/5);  total (14/5)

GGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATG  >  NC_000913/988454‑988541                                              |                                           ggAACATTTCGCCTGCACCTTCGGCATCAGATGCGGTAATCAGTGACGt                                         >  1:862272/1‑49 (MQ=39)  gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                        >  1:712416/1‑49 (MQ=255)       tttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAc                                   >  1:867445/1‑49 (MQ=255)          cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAccc                                 <  1:2154573/48‑1 (MQ=255)           gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                  >  1:975198/1‑46 (MQ=255)           gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                  >  1:1701489/1‑46 (MQ=255)           gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                  >  1:1067181/1‑46 (MQ=255)           gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                  >  1:310650/1‑46 (MQ=255)            ccTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCaga                              <  1:861745/49‑1 (MQ=255)                   cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATcc                       >  1:1577704/1‑49 (MQ=255)                      cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                     <  1:2050191/48‑1 (MQ=255)                      cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                     <  1:625126/48‑1 (MQ=255)                      cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                    >  1:1561432/1‑49 (MQ=255)                      cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                    >  1:870958/1‑49 (MQ=255)                         tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc                 >  1:1843822/1‑49 (MQ=255)                               aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaaa           <  1:1679061/49‑1 (MQ=255)                                   ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaag       >  1:1708708/1‑49 (MQ=255)                                          aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg  >  1:1534935/1‑47 (MQ=255)                                          aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg  >  1:1392767/1‑47 (MQ=255)                                              |                                           GGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATG  >  NC_000913/988454‑988541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑