Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,362	C→T	A84V (GCC→GTC)	mltG →	endolytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,362	0	C	T	100.0%	74.1 / NA	19	A84V (GCC→GTC)	mltG	endolytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/9);  total (10/9)

TCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCT  >  NC_000913/1154318‑1154407                                             |                                              tCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCgg                                            >  1:1025308/1‑48 (MQ=255)       gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTa                                      <  1:1607009/48‑1 (MQ=255)        ctgctgCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                    <  1:989709/49‑1 (MQ=255)          gctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGc                                  >  1:2166880/1‑49 (MQ=255)          gctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGc                                  >  1:2250604/1‑49 (MQ=255)           ctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCg                                   <  1:1878417/47‑1 (MQ=255)           ctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCt                                 <  1:1482003/49‑1 (MQ=255)                    gAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                         <  1:2046778/48‑1 (MQ=255)                      aCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAg                      <  1:1808006/49‑1 (MQ=255)                       ccGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                         >  1:324564/1‑45 (MQ=255)                       ccGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                         >  1:604252/1‑45 (MQ=255)                        cGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                    <  1:2001923/49‑1 (MQ=255)                           aTCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAc                 >  1:451405/1‑49 (MQ=255)                           aTCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAc                 >  1:753343/1‑49 (MQ=255)                            tCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAcc                >  1:248190/1‑49 (MQ=255)                               ttCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTg             >  1:544085/1‑49 (MQ=255)                                 ctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgc            <  1:271605/48‑1 (MQ=255)                                       ttAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGAt     >  1:18003/1‑49 (MQ=255)                                          aaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCt  <  1:1282468/49‑1 (MQ=255)                                             |                                              TCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCT  >  NC_000913/1154318‑1154407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑