Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,240,439	C→T	V60V (GTC→GTT)  D545N (GAC→AAC)	ymgM → cvrA ←	protein YmgM putative K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,240,439	0	C	T	100.0%	55.2 / NA	15	V60V (GTC→GTT)  D545N (GAC→AAC)	ymgM cvrA	protein YmgM putative K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/7);  total (8/7)

CTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCA  >  NC_000913/1240394‑1240482                                              |                                            ctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTAcc                                          >  1:1824170/1‑49 (MQ=255)   ttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGa                                        >  1:64571/1‑49 (MQ=255)    tctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCg                                         <  1:959658/47‑1 (MQ=255)      tcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACaa                                     >  1:1460812/1‑49 (MQ=255)        ggCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAAcc                                   >  1:1391886/1‑49 (MQ=255)                      tcatcCCGGCAAACTCTACCTGGTTACCGACAGCCGGTGCCGCGCCTaa                     <  1:615738/49‑1 (MQ=255)                      tcatcCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTaa                     <  1:431677/49‑1 (MQ=255)                        atcCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTaac                    <  1:1118471/48‑1 (MQ=255)                        atcCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTaac                    >  1:1745526/1‑48 (MQ=255)                        atcCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTaac                    <  1:1897908/48‑1 (MQ=255)                        atcCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTaac                    >  1:998753/1‑48 (MQ=255)                              gCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActgct             >  1:2106957/1‑49 (MQ=255)                                aaaCTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTgg           <  1:162107/49‑1 (MQ=255)                                        ccTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAAtt     <  1:1267545/47‑1 (MQ=255)                                         cTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCa  >  1:1911336/1‑49 (MQ=255)                                              |                                            CTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCA  >  NC_000913/1240394‑1240482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑