Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	92.8 / NA	24	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/8);  total (16/8)

ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGA  >  NC_000913/1270765‑1270856                                               |                                              atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             >  1:629353/1‑49 (MQ=255)      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                        >  1:2311624/1‑49 (MQ=255)        aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                      >  1:263049/1‑49 (MQ=255)        aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                      >  1:2036097/1‑49 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                      <  1:2049643/48‑1 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                     <  1:944884/49‑1 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                     >  1:1770071/1‑49 (MQ=255)              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                <  1:2277661/49‑1 (MQ=255)              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                >  1:821307/1‑49 (MQ=255)                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:1948950/1‑47 (MQ=255)                     tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:1883959/1‑49 (MQ=255)                     tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         <  1:270673/49‑1 (MQ=255)                      gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:671258/1‑48 (MQ=255)                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:1677274/1‑45 (MQ=255)                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:282862/1‑45 (MQ=255)                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:470575/1‑45 (MQ=255)                          gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat                    <  1:720252/49‑1 (MQ=255)                            tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                  <  1:1202838/49‑1 (MQ=255)                                 tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:430935/1‑48 (MQ=255)                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa         >  1:356154/1‑49 (MQ=255)                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        >  1:326808/1‑49 (MQ=255)                                       aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt       <  1:1649689/49‑1 (MQ=255)                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa    >  1:959947/1‑47 (MQ=255)                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga  <  1:1129532/49‑1 (MQ=255)                                               |                                              ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGA  >  NC_000913/1270765‑1270856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑