Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	91.0 / NA	23	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/14);  total (9/14)

GCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTG  >  NC_000913/1382510‑1382600                                              |                                              gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                             <  1:2158865/48‑1 (MQ=255)  cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCa                                           <  1:807290/49‑1 (MQ=255)  cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCa                                           <  1:338639/49‑1 (MQ=255)    aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                          >  1:455505/1‑48 (MQ=255)    aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                         <  1:1147955/49‑1 (MQ=255)        aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                     <  1:1146059/49‑1 (MQ=255)          cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                   >  1:279581/1‑49 (MQ=255)            attatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                   >  1:425358/1‑47 (MQ=255)             ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                >  1:728388/1‑49 (MQ=255)                tctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                              <  1:544867/48‑1 (MQ=255)                 ctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtttt                            >  1:813445/1‑49 (MQ=255)                 ctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtttt                            >  1:1953494/1‑49 (MQ=255)                       cgcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                       >  1:334671/1‑48 (MQ=255)                        gcgGCTTCAATAAGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCa                     <  1:2114841/49‑1 (MQ=255)                            cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                  >  1:441794/1‑48 (MQ=255)                              tCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt               >  1:762468/1‑49 (MQ=255)                               cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt               <  1:2295960/48‑1 (MQ=255)                                    tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc         <  1:783109/49‑1 (MQ=255)                                      gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCt       <  1:533495/49‑1 (MQ=255)                                       acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa      <  1:2021892/49‑1 (MQ=255)                                         gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt    <  1:762257/49‑1 (MQ=255)                                           cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg  <  1:430624/49‑1 (MQ=255)                                           cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg  <  1:543758/49‑1 (MQ=255)                                              |                                              GCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTG  >  NC_000913/1382510‑1382600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑