Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,562,085	C→T	A133T (GCA→ACA)	ddpA ←	putative D,D‑dipeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,562,085	0	C	T	100.0%	95.8 / NA	25	A133T (GCA→ACA)	ddpA	putative D,D‑dipeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/11);  total (14/11)

ACCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGCTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAA  >  NC_000913/1562039‑1562129                                               |                                             aCCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGttt                                            >  1:1421797/1‑49 (MQ=255)    gTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctg                                         >  1:1016126/1‑49 (MQ=255)     tATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgc                                        <  1:1478975/49‑1 (MQ=255)       tGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgctg                                      <  1:569571/49‑1 (MQ=255)           cgtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGccc                                  >  1:49923/1‑49 (MQ=255)            gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGcccc                                 <  1:1065127/49‑1 (MQ=255)            gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGccc                                  <  1:2206612/48‑1 (MQ=255)             tcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                                <  1:109781/49‑1 (MQ=255)             tcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                                <  1:1023091/49‑1 (MQ=255)                     aTCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtt                        >  1:1174780/1‑49 (MQ=255)                        aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAttttt                     <  1:826087/49‑1 (MQ=255)                        aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                      >  1:1282944/1‑48 (MQ=255)                        aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                      >  1:1094323/1‑48 (MQ=255)                           cTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtag                   >  1:1139993/1‑48 (MQ=255)                              tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagtag                <  1:724980/48‑1 (MQ=255)                               aaaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGcc              >  1:2242530/1‑49 (MQ=255)                                aaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGcc              <  1:1869754/48‑1 (MQ=255)                                aaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCg             <  1:461047/49‑1 (MQ=255)                                     ttGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCa         >  1:1919673/1‑48 (MQ=255)                                      tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa        >  1:759869/1‑48 (MQ=255)                                       gggAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa        >  1:882057/1‑47 (MQ=255)                                       gggAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa        >  1:909490/1‑47 (MQ=255)                                          aaaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaaga    >  1:1750445/1‑48 (MQ=255)                                           aaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaagaaa  <  1:820609/49‑1 (MQ=255)                                           aaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaaga    >  1:2126498/1‑47 (MQ=255)                                           aaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaaga    >  1:1383921/1‑47 (MQ=255)                                               |                                             ACCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGCTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAA  >  NC_000913/1562039‑1562129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑