Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,626,996	C→T	D129N (GAT→AAT)	dcp ←	peptidyl‑dipeptidase Dcp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,626,996	0	C	T	100.0%	65.6 / NA	18	D129N (GAT→AAT)	dcp	peptidyl‑dipeptidase Dcp
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/8);  total (10/8)

AAACGTTGATGAATCACCTCCACCAGGCGGATGGATTCACTATCAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCATCTACCCGCGC  >  NC_000913/1626953‑1627041                                            |                                              aaaCGTTGATGAATCACCTCCACCAGGCGGATGGATTCACTATTAAGcc                                          <  1:1662517/49‑1 (MQ=255)     gTTGATGAATCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAg                                      >  1:134266/1‑49 (MQ=255)        gatgaATCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAggg                                    <  1:641458/48‑1 (MQ=255)        gatgaATCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAggg                                    <  1:2363498/48‑1 (MQ=255)         atgaATCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAGGGa                                   <  1:618684/48‑1 (MQ=255)          tgaATCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAGGGAtt                                 <  1:623418/49‑1 (MQ=255)           gaATCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAGGGAtt                                 >  1:2139364/1‑48 (MQ=255)                  cTCCACCAGGCGGATGGATTCACTATTAAGCCCCAGGGATTCACGgcgc                         >  1:858745/1‑49 (MQ=255)                   tCCACCAGGCGGATGGATTCACTATTAAGCCCCAGGGATTCACGGCGCt                        >  1:1802539/1‑49 (MQ=255)                      accaGGCGGATGGATTCACTATTAAGCCCCAGGGATTCACGGCGCTGcc                     <  1:507681/49‑1 (MQ=255)                       ccaGGCGGATGGATTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCa                    <  1:1913354/49‑1 (MQ=255)                        caGGCGGATGGATTCACTATTAAGCCCCAGGGATTCACGGCGCTGc                      >  1:802587/1‑46 (MQ=255)                          ggCGGATGGATTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGa                  >  1:1602771/1‑48 (MQ=255)                            cGGATGGATTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGACAg               >  1:1421225/1‑49 (MQ=255)                            cGGAAGGATTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGACAg               >  1:721980/1‑49 (MQ=39)                             ggatggatTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGACAg               >  1:1525972/1‑48 (MQ=255)                                       aCTATTAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCATCTACCcgc    <  1:235423/49‑1 (MQ=255)                                         tATTAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCATCTACCcgcgc  >  1:247315/1‑49 (MQ=255)                                            |                                              AAACGTTGATGAATCACCTCCACCAGGCGGATGGATTCACTATCAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCATCTACCCGCGC  >  NC_000913/1626953‑1627041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑