Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,028	T→C	V99A (GTC→GCC)	ampD →	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,028	0	T	C	100.0%	52.4 / NA	16	V99A (GTC→GCC)	ampD	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/7);  total (9/7)

GTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGG  >  NC_000913/118985‑119073                                            |                                              gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                           <  1:1776952/48‑1 (MQ=255)    cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                        >  1:421537/1‑48 (MQ=255)    cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                        >  1:2069218/1‑48 (MQ=255)     aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                      <  1:217250/49‑1 (MQ=255)            tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                               >  1:1429643/1‑49 (MQ=255)                   gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                        <  1:1387474/49‑1 (MQ=255)                          gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa                 >  1:1113829/1‑49 (MQ=255)                          gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa                 >  1:1678542/1‑49 (MQ=255)                           tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                <  1:778763/49‑1 (MQ=255)                             catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATg               <  1:395037/48‑1 (MQ=255)                             catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa              >  1:650258/1‑49 (MQ=255)                              atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt             <  1:1281227/49‑1 (MQ=255)                                      cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt     <  1:1120626/49‑1 (MQ=255)                                       gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAtt    >  1:1131286/1‑49 (MQ=255)                                        ggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg   <  1:433715/49‑1 (MQ=255)                                         gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTgg  >  1:1772082/1‑49 (MQ=255)                                         gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTgg  >  1:1706386/1‑49 (MQ=255)                                            |                                              GTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGG  >  NC_000913/118985‑119073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑