Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,662,872	C→T	R773W (CGG→TGG)	ynfF →	putative selenate reductase YnfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,662,872	0	C	T	100.0%	110.0 / NA	28	R773W (CGG→TGG)	ynfF	putative selenate reductase YnfF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/15);  total (13/15)

CGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCG  >  NC_000913/1662826‑1662915                                               |                                            cGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTgg                                           <  1:1168673/49‑1 (MQ=255) cGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTgg                                           >  1:1125026/1‑49 (MQ=255)        gggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGAt                                     >  1:1254701/1‑48 (MQ=255)        gggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGAt                                     <  1:383816/48‑1 (MQ=255)          gCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAc                                  >  1:2271094/1‑49 (MQ=255)          gCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAc                                  <  1:206944/49‑1 (MQ=255)          gCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAc                                  <  1:62002/49‑1 (MQ=255)          gCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAc                                  <  1:1170023/49‑1 (MQ=255)            aaGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcgg                                <  1:372646/49‑1 (MQ=255)             aGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggc                               <  1:77404/49‑1 (MQ=255)               gTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggcgg                             <  1:682596/49‑1 (MQ=255)               gTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggcgg                             >  1:1436500/1‑49 (MQ=255)                  cGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAg                          >  1:1283747/1‑49 (MQ=255)                   gTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGt                         <  1:682382/49‑1 (MQ=255)                    tGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTa                        <  1:1972658/49‑1 (MQ=255)                    tGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTa                        >  1:1201127/1‑49 (MQ=255)                     ggCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTAt                       >  1:1842429/1‑49 (MQ=255)                      gCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTAt                       <  1:203952/48‑1 (MQ=255)                       cTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATc                      >  1:34652/1‑48 (MQ=255)                            aGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATAtt                >  1:1827756/1‑49 (MQ=255)                              cGGATATGTTTGGTGACTGGGTCGATCACGGTGGCAGTATCAATATTCt              <  1:946580/49‑1 (MQ=38)                              cGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCt              <  1:698518/49‑1 (MQ=255)                               ggATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCt              >  1:1997089/1‑48 (MQ=255)                               ggATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCt              >  1:1707807/1‑48 (MQ=255)                                    tGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACctc        <  1:894229/49‑1 (MQ=255)                                       ttGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCa     >  1:2052908/1‑49 (MQ=255)                                         ggTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCAc    <  1:1808606/48‑1 (MQ=255)                                          gTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCg  >  1:1778093/1‑49 (MQ=255)                                               |                                            CGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCG  >  NC_000913/1662826‑1662915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑