Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,697,216	T→C	intergenic (‑164/+57)	uidR ← / ← hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,697,216	0	T	C	100.0%	123.1 / NA	31	intergenic (‑164/+57)	uidR/hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/14);  total (17/14)

TTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGT  >  NC_000913/1697171‑1697261                                              |                                              ttAACCAATAGCCGTGATTTGGCAGCAAGTTATTTAGCAGAGACGCtga                                            >  1:1744989/1‑49 (MQ=255)  tAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtgat                                           >  1:809411/1‑49 (MQ=255)     ccAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAg                                        <  1:2035712/49‑1 (MQ=255)       aaTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGtt                                      >  1:1687168/1‑49 (MQ=255)           gCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTAt                                   <  1:1532018/48‑1 (MQ=255)             cGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATccc                                <  1:1385024/49‑1 (MQ=255)                 aTTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGt                             >  1:1761532/1‑48 (MQ=255)                 aTTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGt                             <  1:187405/48‑1 (MQ=255)                       cTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaacaa                       >  1:2164199/1‑48 (MQ=255)                        tGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAacaaca                     >  1:329944/1‑49 (MQ=255)                         gCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAacac                    >  1:1621766/1‑49 (MQ=255)                         gCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAacac                    >  1:1654317/1‑49 (MQ=255)                          ccAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAacac                    <  1:1395931/48‑1 (MQ=255)                           cAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgc                  <  1:2113593/49‑1 (MQ=255)                           cAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacg                   >  1:2069666/1‑48 (MQ=255)                            aGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgca                 >  1:264595/1‑49 (MQ=255)                             gTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac                <  1:1780955/49‑1 (MQ=255)                             gTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac                >  1:1298970/1‑49 (MQ=255)                                aTTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACgg              <  1:869706/48‑1 (MQ=255)                                aTTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgt             >  1:1805499/1‑49 (MQ=255)                                 tttAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgtt            >  1:905661/1‑49 (MQ=255)                                 tttAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgtt            <  1:1239757/49‑1 (MQ=255)                                   tAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttgt          >  1:2097075/1‑49 (MQ=255)                                   tAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttgt          >  1:955402/1‑49 (MQ=255)                                     gCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttgtt         <  1:1554781/48‑1 (MQ=255)                                      cAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTa       <  1:1915485/49‑1 (MQ=255)                                        gagaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAcc     >  1:196486/1‑49 (MQ=255)                                        gagaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAcc     >  1:122987/1‑49 (MQ=255)                                          gaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCgg   <  1:220638/49‑1 (MQ=255)                                          gaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCgg   <  1:1681338/49‑1 (MQ=255)                                           aCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGt  <  1:2002043/49‑1 (MQ=255)                                              |                                              TTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGT  >  NC_000913/1697171‑1697261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑