Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	77.9 / NA	21	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (13/8);  total (13/8)

GCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAC  >  NC_000913/1742710‑1742798                                              |                                            gcgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                                          >  1:1252954/1‑49 (MQ=255)  cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                         <  1:415331/49‑1 (MQ=255)        gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                   >  1:1292377/1‑49 (MQ=255)               cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                             <  1:23759/48‑1 (MQ=255)                  cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                         >  1:612945/1‑49 (MQ=255)                  cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                            <  1:970949/46‑1 (MQ=255)                   ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                         <  1:1465012/48‑1 (MQ=255)                    cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                       >  1:533632/1‑49 (MQ=255)                    cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                       >  1:431215/1‑49 (MQ=255)                      agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                     >  1:1440543/1‑49 (MQ=255)                          tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                 <  1:1577785/49‑1 (MQ=255)                            gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg               >  1:851292/1‑49 (MQ=255)                               cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa            >  1:378301/1‑49 (MQ=255)                                 ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt          >  1:1135743/1‑49 (MQ=255)                                 ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt          >  1:1638695/1‑49 (MQ=255)                                  tCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt         >  1:1035698/1‑49 (MQ=255)                                    cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc        <  1:639351/48‑1 (MQ=255)                                    cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg       >  1:18257/1‑49 (MQ=255)                                      gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc     <  1:1742839/49‑1 (MQ=255)                                       tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCg    <  1:124814/49‑1 (MQ=255)                                         tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc  >  1:425211/1‑49 (MQ=255)                                              |                                            GCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAC  >  NC_000913/1742710‑1742798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑