Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,533	G→A	G316D (GGC→GAC)	ydiF →	putative acetate‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,533	0	G	A	100.0%	97.0 / NA	25	G316D (GGC→GAC)	ydiF	putative acetate‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/16);  total (9/16)

AAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAC  >  NC_000913/1776488‑1776578                                              |                                              aaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCt                                            <  1:1081701/49‑1 (MQ=255) aaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCt                                            >  1:300887/1‑49 (MQ=255)  aGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTg                                           <  1:632642/49‑1 (MQ=255)   ggCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTgg                                          <  1:350156/49‑1 (MQ=255)     cgcgGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTc                                        <  1:1702679/49‑1 (MQ=255)      gcgGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCg                                       <  1:1537848/49‑1 (MQ=255)        ggTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGcc                                     >  1:189620/1‑49 (MQ=255)         gTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGccc                                    >  1:2262601/1‑49 (MQ=255)             ggAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCgaga                                <  1:1277780/49‑1 (MQ=255)              gAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCgag                                 >  1:2271958/1‑47 (MQ=255)                aTGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGagaag                              >  1:1367775/1‑48 (MQ=255)                   tCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGaagaag                           <  1:1079355/48‑1 (MQ=255)                         tCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGc                    >  1:1055866/1‑49 (MQ=255)                         tCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGc                    <  1:1920999/49‑1 (MQ=255)                           ggTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCt                   <  1:138689/48‑1 (MQ=255)                               ttGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgatg               <  1:1548949/48‑1 (MQ=255)                                tGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGAc             >  1:862876/1‑49 (MQ=255)                                 gCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGAc             <  1:1632730/48‑1 (MQ=255)                                  cTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACtt           <  1:325275/49‑1 (MQ=255)                                   tGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACtt           >  1:1609792/1‑48 (MQ=255)                                     aCGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTa         <  1:1577207/48‑1 (MQ=255)                                      cGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTAtt       <  1:794491/49‑1 (MQ=255)                                        gCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCt     <  1:1322702/49‑1 (MQ=255)                                          aTTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGa   <  1:134264/49‑1 (MQ=255)                                           ttGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAc  >  1:1648874/1‑49 (MQ=255)                                              |                                              AAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAC  >  NC_000913/1776488‑1776578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑