Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,811,208	T→C	V92A (GTT→GCT)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,811,208	0	T	C	100.0%	81.6 / NA	22	V92A (GTT→GCT)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/13);  total (9/13)

AATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTT  >  NC_000913/1811164‑1811249                                             |                                          aaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGcc                                       <  1:585859/49‑1 (MQ=255)      tCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                  >  1:787360/1‑49 (MQ=255)      tCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                  >  1:160505/1‑49 (MQ=255)      tCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                  <  1:398504/49‑1 (MQ=255)        gTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTg                                 >  1:1124886/1‑48 (MQ=255)         ttATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCa                               <  1:131619/49‑1 (MQ=255)             gCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAAc                           >  1:2182394/1‑49 (MQ=255)              ccGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                          <  1:1940967/49‑1 (MQ=255)                  tAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtc                      >  1:1790136/1‑49 (MQ=255)                    gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtctc                    <  1:1235491/49‑1 (MQ=255)                         cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAg                  <  1:1765784/46‑1 (MQ=255)                         cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGtt                <  1:936329/48‑1 (MQ=255)                         cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGtt                <  1:2328958/48‑1 (MQ=255)                           ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg             <  1:1589338/49‑1 (MQ=255)                             tctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGc            >  1:715795/1‑48 (MQ=255)                                 ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt       <  1:2051288/49‑1 (MQ=255)                                   cTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt       >  1:1710773/1‑47 (MQ=255)                                   cTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt       >  1:927529/1‑47 (MQ=255)                                   cTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATc      <  1:2066637/48‑1 (MQ=255)                                    tGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAg    >  1:1049751/1‑49 (MQ=255)                                      aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCa     <  1:2095378/46‑1 (MQ=255)                                      aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtt  <  1:1259260/49‑1 (MQ=255)                                             |                                          AATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTT  >  NC_000913/1811164‑1811249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑