Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,898,152	G→A	A407A (GCG→GCA)	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,898,152	0	G	A	100.0%	71.7 / NA	19	A407A (GCG→GCA)	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/12);  total (7/12)

CATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGA  >  NC_000913/1898107‑1898196                                              |                                             cATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACgg                                           <  1:411633/49‑1 (MQ=255) cATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACgg                                           <  1:360221/49‑1 (MQ=255)  aTTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGa                                          >  1:2211068/1‑49 (MQ=255)      aGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATgg                                       <  1:1649768/48‑1 (MQ=255)       gCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGct                                     <  1:708283/49‑1 (MQ=255)       gCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGct                                     >  1:498656/1‑49 (MQ=255)         gTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGctct                                   <  1:351602/49‑1 (MQ=255)          tAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTg                                  >  1:616308/1‑49 (MQ=255)                cATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGcgccgc                            <  1:479750/49‑1 (MQ=255)                      ctctGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGt                      >  1:343855/1‑49 (MQ=255)                      ctctGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGt                      >  1:1640478/1‑49 (MQ=255)                         tgtgAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTg                     <  1:224810/47‑1 (MQ=255)                         tgtgAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCa                   >  1:492706/1‑49 (MQ=255)                             aaGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACcgc               <  1:731693/49‑1 (MQ=255)                               ggCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACcgcgc             <  1:2294046/49‑1 (MQ=255)                                      aaCGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCg       <  1:2357734/48‑1 (MQ=255)                                      aaCGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCg       <  1:1849645/48‑1 (MQ=255)                                        cgccgcACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTg    <  1:1966562/49‑1 (MQ=255)                                          ccgcACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGa  >  1:1974707/1‑49 (MQ=255)                                              |                                             CATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGA  >  NC_000913/1898107‑1898196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑