Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,907,399	C→T	E13K (GAG→AAG)	cspC ←	stress protein, member of the CspA family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,907,399	0	C	T	100.0%	103.6 / NA	27	E13K (GAG→AAG)	cspC	stress protein, member of the CspA family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/11);  total (16/11)

CTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAAT  >  NC_000913/1907359‑1907444                                         |                                              ctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTTGTTGAAcc                                       <  1:1536767/49‑1 (MQ=255) ctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTTGTTGAAc                                        <  1:2075771/48‑1 (MQ=255)      aTCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTTGTTGAACCACTTa                                  <  1:2244168/49‑1 (MQ=255)         aGCCGGAGTAATGAAGCCAAAACCTTTAGACTTGTTGAACCACTTAAc                                <  1:1353840/48‑1 (MQ=255)          gCCGGAGTAATGAAGCCAAAACCTTTAGACTTGTTGAACCACTTAACCt                              >  1:2243202/1‑49 (MQ=255)            cGGAGTAATGAAGCCAAAACCTTTAGACTTGTTGAACCACTTAACCTGa                            <  1:752/49‑1 (MQ=255)            cGGAGTAATGAAGCCAAAACCTTTAGACTTGTTGAACCACTTAACCTGa                            >  1:210009/1‑49 (MQ=255)             ggAGTAATGAAGCCAAAACCTTTAGACTTGTTGAACCACTTAACCTGAc                           >  1:823891/1‑49 (MQ=255)              gAGTAATGAAGCCAAAACCTTTAGACTTGTTGATCCACTTAACCTGAcc                          >  1:808999/1‑49 (MQ=37)                gTAATGAAGCCAAAACCTTTAGACTTGTTGAACCACTTAACCTGACCtt                        >  1:214041/1‑49 (MQ=255)                gTAATGAAGCCAAAACCTTTAGACTTGTTGAACCACTTAACCTGACCtt                        >  1:1234999/1‑49 (MQ=255)                 tAATGAAGCCAAAACCTTTAGACTTGTTGAACCACTTAACCTGACCttt                       <  1:12907/49‑1 (MQ=255)                 tAATGAAGCCAAAACCTTTAGACTTGTTGAACCACTTAACCTGACCttt                       <  1:1283269/49‑1 (MQ=255)                 tAATGAAGCCAAAACCTTTAGACTTGTTGAACCACTTAACCTGACCtt                        >  1:1279831/1‑48 (MQ=255)                  aaTGAAGCCAAAACCTTTAGACTTGTTGAACCACTTAACCTGACCttt                       >  1:2309573/1‑48 (MQ=255)                      aaGCCAAAACCTTTAGACTTGTTGAACCACTTAACCTGACCTTTAATCt                  <  1:191351/49‑1 (MQ=255)                        gCCAAAACCTTTAGACTTGTTGAACCACTTAACCTGACCTTTAATCttt                <  1:622650/49‑1 (MQ=255)                          cAAAACCTTTAGACTTGTTGAACCACTTAACCTGACCTTTAATCTTTGc              >  1:1067621/1‑49 (MQ=255)                          cAAAACCTTTAGACTTGTTGAACCACTTAACCTGACCTTTAATCTTTGc              >  1:2278425/1‑49 (MQ=255)                              aCCTTTAGACTTGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt          <  1:1308005/49‑1 (MQ=255)                               ccTTTAGACTTGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAttt         >  1:1546071/1‑49 (MQ=255)                                cTTTAGACTTGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAttt         >  1:124616/1‑48 (MQ=255)                                cTTTAGACTTGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAttt         >  1:2174722/1‑48 (MQ=255)                                  ttAGACTTGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGa       >  1:2229834/1‑48 (MQ=255)                                   tAGACTTGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaa      >  1:1571227/1‑48 (MQ=255)                                     gACTTGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaa    <  1:353281/48‑1 (MQ=255)                                      aCTTGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAAt  >  1:1176388/1‑49 (MQ=255)                                         |                                              CTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAAT  >  NC_000913/1907359‑1907444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑