Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,784	A→G	V267A (GTC→GCC)	znuA ←	Zn(2(+)) ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,784	0	A	G	100.0%	41.9 / NA	12	V267A (GTC→GCC)	znuA	Zn(2(+)) ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/5);  total (7/5)

AGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAAC  >  NC_000913/1941742‑1941827                                           |                                            aGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGctct                                       >  1:2153250/1‑49 (MQ=255)    ggATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCaa                                    >  1:220235/1‑49 (MQ=255)        ccAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGAc                                >  1:1633617/1‑49 (MQ=255)           aCGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGc                             >  1:1060514/1‑49 (MQ=255)                 ccATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCt                       >  1:1637716/1‑49 (MQ=255)                       gAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACtg                 >  1:823294/1‑49 (MQ=255)                           ggATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGc             <  1:509974/49‑1 (MQ=255)                                tCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAg         <  1:703503/48‑1 (MQ=255)                                 cccTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGc        >  1:759226/1‑48 (MQ=255)                                   cTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCaaa     <  1:253791/49‑1 (MQ=255)                                   cTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCaaa     <  1:532345/49‑1 (MQ=255)                                      gTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAAc  <  1:334760/49‑1 (MQ=255)                                           |                                            AGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAAC  >  NC_000913/1941742‑1941827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑