Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,040,801	T→C	L107L (TTA→CTA)	msrQ →	periplasmic protein‑L‑methionine sulfoxide reductase heme binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,040,801	0	T	C	100.0%	57.7 / NA	16	L107L (TTA→CTA)	msrQ	periplasmic protein‑L‑methionine sulfoxide reductase heme binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/6);  total (10/6)

TAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGTTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAGG  >  NC_000913/2040757‑2040844                                             |                                            tAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGctat                                          <  1:875903/48‑1 (MQ=255)  aaCAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGctatt                                         <  1:1915284/48‑1 (MQ=255)  aaCAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGctat                                          >  1:1594943/1‑47 (MQ=255)      aGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTa                                    <  1:1821669/49‑1 (MQ=255)          aCGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAgg                                 <  1:128037/48‑1 (MQ=255)           cGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGa                                <  1:1617467/48‑1 (MQ=255)           cGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAg                               >  1:1963350/1‑49 (MQ=255)               ttGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTaa                           >  1:1483181/1‑49 (MQ=255)                        cTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGc                  >  1:1283974/1‑49 (MQ=255)                         tGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGcc                 >  1:584749/1‑49 (MQ=255)                           ggCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCt                <  1:1389352/48‑1 (MQ=255)                            gCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTa              <  1:625223/49‑1 (MQ=255)                               tGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTAtt            >  1:1304775/1‑48 (MQ=255)                                 aataatCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTaa         >  1:2345876/1‑49 (MQ=255)                                 aataatCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTa          <  1:2211380/48‑1 (MQ=255)                                  ataatCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAAc        >  1:44815/1‑49 (MQ=255)                                  ataatCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAAc        >  1:747857/1‑49 (MQ=255)                                    aatCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGt      >  1:784363/1‑49 (MQ=255)                                        tGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAgg  >  1:343091/1‑49 (MQ=255)                                          gcgcTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAg   <  1:1733992/46‑1 (MQ=255)                                             |                                            TAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGTTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAGG  >  NC_000913/2040757‑2040844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑