Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,885	C→T	T18I (ACC→ATC)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,885	0	C	T	100.0%	66.3 / NA	18	T18I (ACC→ATC)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/8);  total (10/8)

TGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAG  >  NC_000913/214840‑214928                                              |                                            tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg                                           <  1:1585331/48‑1 (MQ=255)      tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAtt                                      <  1:850402/48‑1 (MQ=255)       cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc                                     <  1:9744/48‑1 (MQ=255)         cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                  <  1:1980591/49‑1 (MQ=255)           aCATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                >  1:827733/1‑49 (MQ=255)               gTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                            >  1:1549024/1‑49 (MQ=255)                 tGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                          >  1:429442/1‑49 (MQ=255)                  gCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgcg                         >  1:658426/1‑49 (MQ=255)                  gCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                          <  1:2231041/48‑1 (MQ=255)                    tATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                       >  1:104334/1‑49 (MQ=255)                     aTTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg                      >  1:1067722/1‑49 (MQ=255)                         ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg                  >  1:1431526/1‑49 (MQ=255)                           gATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                 >  1:1943906/1‑48 (MQ=255)                               gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagc             <  1:1394799/48‑1 (MQ=255)                                tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCAt           <  1:2250471/49‑1 (MQ=255)                                 gAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg          >  1:1025739/1‑49 (MQ=255)                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt         >  1:624431/1‑49 (MQ=255)                                         gaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTaagaag  <  1:992779/49‑1 (MQ=38)                                              |                                            TGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAG  >  NC_000913/214840‑214928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑