Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,662,004	T→C	E43G (GAA→GGA)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,662,004	0	T	C	100.0%	76.7 / NA	20	E43G (GAA→GGA)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/10);  total (10/10)

GCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAG  >  NC_000913/2661961‑2662046                                            |                                           gCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGa                                       <  1:2354317/49‑1 (MQ=255)   tGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                      >  1:860366/1‑48 (MQ=255)     gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAg                                   <  1:445018/49‑1 (MQ=255)     gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAg                                   <  1:2141950/49‑1 (MQ=255)      aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa                                  <  1:2205732/49‑1 (MQ=255)      aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa                                  <  1:2178548/49‑1 (MQ=255)          cAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg                              >  1:1409885/1‑49 (MQ=255)            gACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGa                            <  1:1268556/49‑1 (MQ=255)               cATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAt                         <  1:995175/49‑1 (MQ=255)                         cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGt               >  1:2117898/1‑49 (MQ=255)                         cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGt               <  1:865250/49‑1 (MQ=255)                          aGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt              <  1:245346/49‑1 (MQ=255)                            aCGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg            >  1:2128018/1‑49 (MQ=255)                            aCGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg            >  1:197060/1‑49 (MQ=255)                              gggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg           >  1:673607/1‑48 (MQ=255)                               ggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa          >  1:56178/1‑48 (MQ=255)                                gAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa        >  1:47377/1‑49 (MQ=255)                                  aaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAata      <  1:634842/49‑1 (MQ=255)                                      aGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAg  >  1:1978584/1‑49 (MQ=255)                                      aGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAg  >  1:1846455/1‑49 (MQ=255)                                            |                                           GCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAG  >  NC_000913/2661961‑2662046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑