Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	257,794	T→C	intergenic (+23/‑35)	frsA → / → crl	fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	257,794	0	T	C	100.0%	77.5 / NA	20	intergenic (+23/‑35)	frsA/crl	fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/9);  total (11/9)

AAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAC  >  NC_000913/257757‑257838                                      |                                             aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                   >  1:526488/1‑49 (MQ=255)     gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGc                               <  1:876250/49‑1 (MQ=255)      cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                              <  1:4346/49‑1 (MQ=255)        tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                             >  1:2134525/1‑48 (MQ=255)          tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac                          <  1:1753737/49‑1 (MQ=255)          tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac                          <  1:392430/49‑1 (MQ=255)           gtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac                          >  1:960190/1‑48 (MQ=255)               aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                     <  1:1200455/49‑1 (MQ=255)               aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                     >  1:742827/1‑49 (MQ=255)                aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                     >  1:1480621/1‑48 (MQ=255)                aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                     >  1:1800059/1‑48 (MQ=255)                aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                     >  1:1928313/1‑48 (MQ=255)                aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                     >  1:448837/1‑48 (MQ=255)                    ttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                 >  1:1161214/1‑48 (MQ=255)                          aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt          >  1:1178124/1‑49 (MQ=255)                          aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt          >  1:796369/1‑49 (MQ=255)                            ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa        <  1:426467/49‑1 (MQ=255)                              ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc       <  1:1389487/48‑1 (MQ=255)                                gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt     <  1:73156/48‑1 (MQ=255)                                  cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc  <  1:509004/49‑1 (MQ=255)                                      |                                             AAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAC  >  NC_000913/257757‑257838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑