Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,052,352	C→A	W389C (TGG→TGT)	ubiH ←	2‑octaprenyl‑6‑methoxyphenol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,052,352	0	C	A	100.0%	140.4 / NA	37	W389C (TGG→TGT)	ubiH	2‑octaprenyl‑6‑methoxyphenol hydroxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/14);  total (23/14)

ACACTTTGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACCCAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCGGGGTGAA  >  NC_000913/3052307‑3052396                                              |                                             acacTTTGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACaa                                           >  1:1634060/1‑49 (MQ=255) acacTTTGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACaa                                           <  1:1616428/49‑1 (MQ=255)   acTTTGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACAAcc                                         >  1:1898018/1‑49 (MQ=255)    cTTTGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACAAcc                                         >  1:1098899/1‑48 (MQ=255)    cTTTGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACAACCg                                        <  1:1888651/49‑1 (MQ=255)    cTTTGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACAACCg                                        <  1:1685952/49‑1 (MQ=255)     tttGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACAAcc                                         >  1:570416/1‑47 (MQ=255)       tGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAgg                                     >  1:847322/1‑49 (MQ=255)        gCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAggg                                    >  1:515454/1‑49 (MQ=255)         cATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGt                                   >  1:227788/1‑49 (MQ=255)             gTTTATTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGtgcgc                               >  1:118999/1‑49 (MQ=255)              tttATTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGtgcgct                              <  1:295759/49‑1 (MQ=255)              tttATTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGtgcgct                              <  1:1125332/49‑1 (MQ=255)                tATTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGcgcgctgc                            >  1:1471656/1‑49 (MQ=255)                 aTTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGtgcgctgcg                           >  1:1176719/1‑49 (MQ=255)                 aTTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGtgcgctgc                            <  1:1242340/48‑1 (MQ=255)                     cTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCa                        <  1:514668/48‑1 (MQ=255)                     cTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAg                       <  1:1083103/49‑1 (MQ=255)                        aaaaCCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGca                     >  1:160645/1‑48 (MQ=255)                        aaaaCCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGc                      <  1:1078489/47‑1 (MQ=255)                         aaaCCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGcaca                   >  1:841018/1‑49 (MQ=255)                         aaaCCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGcaca                   <  1:713544/49‑1 (MQ=255)                          aaCCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACAt                  <  1:1778513/49‑1 (MQ=255)                          aaCCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACAt                  >  1:200123/1‑49 (MQ=255)                           aCCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACATc                 >  1:910907/1‑49 (MQ=255)                             cGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACATcgc               >  1:2191180/1‑49 (MQ=255)                              gCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACATcgcg              >  1:795909/1‑49 (MQ=255)                               ccTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGt             <  1:1579821/49‑1 (MQ=255)                                   cAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCg         >  1:309375/1‑49 (MQ=255)                                   cAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCg         >  1:517383/1‑49 (MQ=255)                                   cAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCg         >  1:1313531/1‑49 (MQ=255)                                   cAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCg         >  1:208046/1‑49 (MQ=255)                                    aaCGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCgg        >  1:1581886/1‑49 (MQ=255)                                      cgcgCCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCgggg      <  1:1998040/49‑1 (MQ=255)                                       gcgcCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCGGGGt     <  1:1449711/49‑1 (MQ=255)                                       gcgcCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCGGGGt     >  1:876009/1‑49 (MQ=255)                                         gcCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCGGGGTg    >  1:1588626/1‑48 (MQ=255)                                           cacacaACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCGGGGTGaa  >  1:2162335/1‑48 (MQ=255)                                              |                                             ACACTTTGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACCCAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCGGGGTGAA  >  NC_000913/3052307‑3052396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑