Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,090,282	C→T	intergenic (+30/‑65)	yggI → / → endA	conserved protein YggI/DNA‑specific endonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,090,282	0	C	T	100.0%	168.5 / NA	43	intergenic (+30/‑65)	yggI/endA	conserved protein YggI/DNA‑specific endonuclease I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/20);  total (23/20)

AGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCG  >  NC_000913/3090236‑3090327                                               |                                              aGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTg                                             >  1:2266552/1‑49 (MQ=255)     ggTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCt                                          >  1:2146994/1‑48 (MQ=255)      gTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGa                                        >  1:414974/1‑49 (MQ=255)        tGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGAtt                                      >  1:694604/1‑49 (MQ=255)         gCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGAtt                                      <  1:1350508/48‑1 (MQ=255)         gCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGAtt                                      <  1:1838885/48‑1 (MQ=255)          cGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTg                                     <  1:262022/48‑1 (MQ=255)          cGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGc                                    <  1:1727480/49‑1 (MQ=255)            aaaTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCa                                   >  1:369724/1‑48 (MQ=255)              aTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAc                                >  1:1477772/1‑49 (MQ=255)              aTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAc                                >  1:2040731/1‑49 (MQ=255)               tAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAcc                               <  1:1253613/49‑1 (MQ=255)                  ccATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaa                            <  1:1165565/49‑1 (MQ=255)                  ccATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaa                            <  1:806375/49‑1 (MQ=255)                  ccATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaa                            <  1:1515225/49‑1 (MQ=255)                  ccATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaa                             >  1:312356/1‑48 (MQ=255)                   cATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                           >  1:1711048/1‑49 (MQ=255)                   cATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                           >  1:879752/1‑49 (MQ=255)                   cATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                           >  1:2272841/1‑49 (MQ=255)                     tCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                           >  1:1841677/1‑47 (MQ=255)                     tCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                           >  1:1968067/1‑47 (MQ=255)                       tGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGc                       >  1:1693407/1‑49 (MQ=255)                       tGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGc                       >  1:132984/1‑49 (MQ=255)                        gAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCt                      >  1:1487530/1‑49 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCtt                     >  1:1742500/1‑49 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCt                      <  1:234693/48‑1 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCt                      <  1:2064429/48‑1 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCt                      <  1:177687/48‑1 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCt                      <  1:1473527/48‑1 (MQ=255)                           cTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTc                   >  1:2247333/1‑49 (MQ=255)                           cTATCAGGAACTTTCCTGATTGGGCTGATTGCATACCAAAACAGCTTTc                   >  1:787045/1‑49 (MQ=39)                            tATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCg                  >  1:553434/1‑49 (MQ=255)                              tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCt                <  1:652406/49‑1 (MQ=255)                              tCAGGAACTTTCCTCATTTGGCTGATTGCATACCAAAACAGCTTTCGc                 <  1:1126133/48‑1 (MQ=39)                                aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTAc              <  1:1630005/49‑1 (MQ=255)                                aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTAc              <  1:919834/49‑1 (MQ=255)                                 ggAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACg             >  1:2258537/1‑49 (MQ=255)                                 ggAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACg             <  1:1605510/49‑1 (MQ=255)                                     cTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGc         >  1:281162/1‑49 (MQ=255)                                        tCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCt        <  1:331166/47‑1 (MQ=255)                                        tCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTg       <  1:540581/48‑1 (MQ=255)                                          cTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGc     <  1:244384/48‑1 (MQ=255)                                            gATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCg  >  1:1951643/1‑49 (MQ=255)                                               |                                              AGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCG  >  NC_000913/3090236‑3090327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑