Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,150	C→T	K90K (AAG→AAA)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,150	0	C	T	100.0%	149.0 / NA	38	K90K (AAG→AAA)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/27);  total (11/27)

ATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTT  >  NC_000913/3477107‑3477193                                            |                                            acgCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                        <  1:68145/47‑1 (MQ=255) aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                         >  1:2026754/1‑48 (MQ=255) aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                        <  1:2013125/49‑1 (MQ=255)    cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                      <  1:2320363/48‑1 (MQ=255)    cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                      <  1:147975/48‑1 (MQ=255)    cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                     <  1:2364815/49‑1 (MQ=255)    cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                     <  1:938451/49‑1 (MQ=255)     ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                     >  1:2170768/1‑48 (MQ=255)     ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                     >  1:209928/1‑48 (MQ=255)      tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                    >  1:1603883/1‑48 (MQ=255)       ttAGAGTCTTTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                     <  1:509473/45‑1 (MQ=255)       gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                    <  1:683984/47‑1 (MQ=255)       gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                    <  1:2100361/47‑1 (MQ=255)          gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                <  1:227447/48‑1 (MQ=255)            gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                             <  1:2170181/49‑1 (MQ=255)               tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                          >  1:755533/1‑49 (MQ=255)                gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                          >  1:2130419/1‑48 (MQ=255)                 ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                          <  1:1737483/47‑1 (MQ=255)                 ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                         <  1:2346002/48‑1 (MQ=255)                 ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa                        <  1:55027/49‑1 (MQ=255)                   cGATCTCTTGGTCGGGGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                       <  1:1471420/48‑1 (MQ=39)                   cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                       >  1:1071769/1‑48 (MQ=255)                       ctctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                    >  1:1211899/1‑47 (MQ=255)                        tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                 >  1:2120966/1‑49 (MQ=255)                          ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                  <  1:2348238/46‑1 (MQ=255)                            ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCg               <  1:1205487/47‑1 (MQ=255)                            ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt             >  1:761533/1‑49 (MQ=255)                             gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt             <  1:2313033/48‑1 (MQ=255)                              tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa           <  1:89325/49‑1 (MQ=255)                              tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa           <  1:1680206/49‑1 (MQ=255)                              tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa           <  1:1704538/49‑1 (MQ=255)                              tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa           <  1:691113/49‑1 (MQ=255)                                ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg          <  1:1332651/48‑1 (MQ=255)                                ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg          <  1:280365/48‑1 (MQ=255)                                  agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg       <  1:1160251/49‑1 (MQ=255)                                    agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt     <  1:2271340/49‑1 (MQ=255)                                      tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt   <  1:695817/49‑1 (MQ=255)                                        tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt  >  1:486013/1‑48 (MQ=255)                                            |                                            ATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTT  >  NC_000913/3477107‑3477193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑