Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,003	A→G	D212D (GAT→GAC)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,003	0	A	G	100.0%	119.5 / NA	31	D212D (GAT→GAC)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/20);  total (11/20)

GAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTC  >  NC_000913/3512959‑3513041                                             |                                       gAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                    <  1:1605293/49‑1 (MQ=255)   gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                                  <  1:905911/49‑1 (MQ=255)    tCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                 <  1:2325856/49‑1 (MQ=255)        tcaCCGCACTTTTTATTTTCTTCACTACCGATTTCGGGTCTTCcag                                <  1:2022879/44‑1 (MQ=37)        tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                             <  1:1497773/49‑1 (MQ=255)         gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                            >  1:119862/1‑49 (MQ=255)          aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                           <  1:2203031/49‑1 (MQ=255)             gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                        >  1:1382501/1‑49 (MQ=255)               aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                       >  1:164483/1‑48 (MQ=255)               aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATa                      <  1:1249505/49‑1 (MQ=255)                cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                     <  1:1767449/49‑1 (MQ=255)                cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                     <  1:2348746/49‑1 (MQ=255)                 gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                    <  1:503265/49‑1 (MQ=255)                 gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                    <  1:2269505/49‑1 (MQ=255)                     gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                >  1:845983/1‑49 (MQ=255)                      aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                <  1:304690/48‑1 (MQ=255)                      aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt               <  1:387671/49‑1 (MQ=255)                       ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt              <  1:206922/49‑1 (MQ=255)                          tcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc            <  1:112648/48‑1 (MQ=255)                           cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCg           <  1:52108/48‑1 (MQ=255)                           cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGa          >  1:855501/1‑49 (MQ=255)                            ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt         >  1:1509737/1‑49 (MQ=255)                              cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt         <  1:2031688/47‑1 (MQ=255)                               aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa       <  1:1035902/48‑1 (MQ=255)                               aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa       <  1:750767/48‑1 (MQ=255)                                cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc     >  1:218890/1‑49 (MQ=255)                                cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc     >  1:1446044/1‑49 (MQ=255)                                 tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCg    >  1:24600/1‑49 (MQ=255)                                   ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTc  <  1:1466024/49‑1 (MQ=255)                                    cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt   >  1:932214/1‑47 (MQ=255)                                     gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt   >  1:2296786/1‑46 (MQ=255)                                             |                                       GAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTC  >  NC_000913/3512959‑3513041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑