Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,527,760	A→G	N431S (AAC→AGC)	igaA →	inner membrane protein ‑ inhibits the Rcs signaling pathway

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,527,760	0	A	G	100.0%	82.5 / NA	22	N431S (AAC→AGC)	igaA	inner membrane protein ‑ inhibits the Rcs signaling pathway
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/12);  total (10/12)

CACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAAC  >  NC_000913/3527721‑3527803                                        |                                            cACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc                                     <  1:359515/48‑1 (MQ=255)  aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGTCGCCCg                                    <  1:2106618/48‑1 (MQ=39)  aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCg                                    <  1:736473/48‑1 (MQ=255)     ttttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa                                >  1:2261045/1‑49 (MQ=255)      tttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAt                               >  1:2072498/1‑49 (MQ=255)       ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt                              >  1:629637/1‑49 (MQ=255)        tttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt                              <  1:977558/48‑1 (MQ=255)        tttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt                              <  1:580423/48‑1 (MQ=255)          tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc                            >  1:146287/1‑48 (MQ=255)          tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc                            >  1:681586/1‑48 (MQ=255)          tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc                            >  1:1966719/1‑48 (MQ=255)          tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc                            <  1:1527190/48‑1 (MQ=255)           aCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCa                          >  1:1389471/1‑49 (MQ=255)            ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt                         <  1:1902805/49‑1 (MQ=255)               tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc                      <  1:1946807/49‑1 (MQ=255)               tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc                      >  1:903699/1‑49 (MQ=255)                  gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc                      >  1:487468/1‑46 (MQ=255)                   aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGa                  <  1:35569/49‑1 (MQ=255)                       cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATc               <  1:1928364/48‑1 (MQ=255)                          gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGa            <  1:2013896/48‑1 (MQ=255)                               tcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt      <  1:936583/49‑1 (MQ=255)                                    tGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaac  >  1:889533/1‑48 (MQ=255)                                        |                                            CACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAAC  >  NC_000913/3527721‑3527803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑