Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,850,891	G→A	Q68* (CAA→TAA)	ilvN ←	acetohydroxy acid synthase I subunit IlvN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,850,891	0	G	A	100.0%	62.3 / NA	17	Q68* (CAA→TAA)	ilvN	acetohydroxy acid synthase I subunit IlvN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/7);  total (10/7)

CTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCC  >  NC_000913/3850847‑3850935                                             |                                             cTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCtt                                          <  1:277235/49‑1 (MQ=255) cTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCt                                           >  1:123391/1‑48 (MQ=255)      tACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatcat                                     >  1:1148003/1‑49 (MQ=255)        cGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatcatc                                    <  1:955518/48‑1 (MQ=255)        cGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCt                                   <  1:413134/49‑1 (MQ=255)         gCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTg                                  <  1:597089/49‑1 (MQ=255)         gCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTg                                  >  1:1843628/1‑49 (MQ=255)                tttCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGa                           >  1:2121778/1‑49 (MQ=255)                      gACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTgg                     >  1:1221613/1‑49 (MQ=255)                              ccAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCAtt             >  1:1472631/1‑49 (MQ=255)                                  cTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAc          >  1:1627026/1‑48 (MQ=255)                                  cTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAc          >  1:1522761/1‑48 (MQ=255)                                     aTCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg       <  1:983483/48‑1 (MQ=255)                                        gATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAg    <  1:97191/48‑1 (MQ=255)                                        gATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc   >  1:274229/1‑49 (MQ=255)                                        gATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc   >  1:1984378/1‑49 (MQ=255)                                         aTTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGcc  <  1:877396/49‑1 (MQ=255)                                             |                                             CTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCC  >  NC_000913/3850847‑3850935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑