Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,696	C→T	G103D (GGC→GAC)	asnC ←	DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,696	0	C	T	100.0%	111.1 / NA	29	G103D (GGC→GAC)	asnC	DNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/12);  total (17/12)

GTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAG  >  NC_000913/3926653‑3926739                                            |                                            gTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGtt                                        >  1:1064337/1‑49 (MQ=255)    gatcgaACGGCACATCGCTTTTATAAAGATGCTGTAGTGGTCGGTtgtg                                     <  1:1632672/49‑1 (MQ=255)     atcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTgtgt                                    <  1:623527/49‑1 (MQ=255)      tcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTa                                   <  1:381989/49‑1 (MQ=255)            ggCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGc                             >  1:102237/1‑49 (MQ=255)            ggCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGc                             >  1:184910/1‑49 (MQ=255)              cacaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                           <  1:1207359/49‑1 (MQ=255)              cacaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                           >  1:331902/1‑49 (MQ=255)              cacaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                           >  1:2214951/1‑49 (MQ=255)               acaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                           >  1:1105174/1‑48 (MQ=255)                caTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCa                         <  1:328191/49‑1 (MQ=255)                 aTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAg                        >  1:713728/1‑49 (MQ=255)                 aTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAg                        >  1:1789008/1‑49 (MQ=255)                  tCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGt                       <  1:972890/49‑1 (MQ=255)                        ttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACt                   <  1:1526276/47‑1 (MQ=255)                         tataAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtca                >  1:2055345/1‑49 (MQ=255)                         tataAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtca                >  1:1290618/1‑49 (MQ=255)                         tataAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtc                 <  1:1765866/48‑1 (MQ=255)                         tataAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtc                 <  1:229713/48‑1 (MQ=255)                          ataAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtca                <  1:1465384/48‑1 (MQ=255)                           taAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcat               >  1:2168656/1‑48 (MQ=255)                            aaaGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatc              >  1:1016583/1‑48 (MQ=255)                             aaGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatca             <  1:1350411/48‑1 (MQ=255)                             aaGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCaa            <  1:523278/49‑1 (MQ=255)                              aGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAg           >  1:1356384/1‑49 (MQ=255)                                  gCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCtt       >  1:1511017/1‑49 (MQ=255)                                     gTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTc     >  1:796198/1‑48 (MQ=255)                                       aGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAg  >  1:1255477/1‑49 (MQ=255)                                       aGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAg  >  1:1182441/1‑49 (MQ=255)                                            |                                            GTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAG  >  NC_000913/3926653‑3926739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑