Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	380,022	(G)10→9	intergenic (‑141/+47)	frmR ← / ← yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	380,013	0	G	.	93.3%	59.4 / ‑0.5	15	intergenic (‑132/+56)	frmR/yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (6/8);  total (7/8)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.54e-01

GAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAG  >  NC_000913/379966‑380052                                                |                                        gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                        <  1:2037006/49‑1 (MQ=255)  agaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                       >  1:2111690/1‑49 (MQ=255)     aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg                                    >  1:2341249/1‑49 (MQ=255)       aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggg                                  >  1:761398/1‑49 (MQ=255)       aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggg                                  >  1:1461832/1‑49 (MQ=255)       aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggg                                  >  1:1541311/1‑49 (MQ=255)         aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggc                                <  1:2633244/49‑2 (MQ=255)               tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCg                         <  1:168363/49‑1 (MQ=255)                 cGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCgg                        >  1:1596311/1‑49 (MQ=255)                 cGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGc                       <  1:1279956/49‑1 (MQ=255)                    tGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAgg                    >  1:221451/1‑49 (MQ=255)                       aTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGaa                 <  1:2045820/49‑1 (MQ=255)                        tgtgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAg                <  1:1745182/49‑1 (MQ=255)                           ggTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgcc             >  1:1084710/1‑49 (MQ=255)                           ggTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgcc             >  1:2719693/1‑49 (MQ=255)                            gTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgcc             <  1:1335289/48‑1 (MQ=255)                             tCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgccgc           >  1:1966107/1‑49 (MQ=255)                               ggAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGccc         >  1:2417719/1‑49 (MQ=255)                                gAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc        >  1:1399396/1‑49 (MQ=255)                                 aaaaCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCg       <  1:308671/49‑1 (MQ=255)                                    aCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAt    <  1:79909/49‑1 (MQ=255)                                      aaaGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAg  <  1:2559434/49‑1 (MQ=255)                                                |                                        GAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAG  >  NC_000913/379966‑380052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑