Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	143.3 / NA	31	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (15/16);  total (15/16)

TTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACA  >  NC_000913/765143‑765232                                               |                                            ttttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                           >  1:1754695/1‑48 (MQ=255)   ttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                           >  1:2171837/1‑46 (MQ=255)      tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                      >  1:569394/1‑48 (MQ=38)       gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTc                                     <  1:422288/48‑1 (MQ=38)       gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTc                                     <  1:1916111/48‑1 (MQ=38)          cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                 >  1:936585/1‑49 (MQ=38)           ttATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                >  1:1988404/1‑49 (MQ=38)             aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                <  1:866631/47‑1 (MQ=38)             aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                <  1:2407269/47‑1 (MQ=38)             aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                               <  1:2112813/48‑1 (MQ=38)              tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                             <  1:2169087/49‑1 (MQ=38)                gCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                           >  1:934221‑M1/1‑49 (MQ=255)                gCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                           >  1:127878‑M1/1‑49 (MQ=255)                 cGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAt                          <  1:1849073‑M1/49‑2 (MQ=255)                 cGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAt                          <  1:1035823‑M1/49‑2 (MQ=255)                  gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                           >  1:127288‑M1/1‑47 (MQ=255)                   atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                        >  1:2322770‑M1/1‑46 (MQ=255)                     gatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                       >  1:527689‑M1/1‑44 (MQ=255)                      atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagca                      <  1:1007125‑M1/48‑6 (MQ=255)                          tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                  <  1:216876‑M1/48‑10 (MQ=255)                          tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                  <  1:1185612‑M1/48‑10 (MQ=255)                          tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                  >  1:1095776‑M1/1‑39 (MQ=255)                           cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                >  1:745731‑M1/1‑38 (MQ=255)                            gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac               <  1:1752784‑M1/49‑13 (MQ=255)                                cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaa           <  1:82196‑M1/49‑17 (MQ=255)                                  cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc         >  1:2294932‑M1/1‑31 (MQ=255)                                  cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc         >  1:666377‑M1/1‑31 (MQ=255)                                  cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc         >  1:2087755‑M1/1‑31 (MQ=255)                                    aTCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca       >  1:1877012‑M1/1‑29 (MQ=255)                                     tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca       <  1:2511454‑M1/48‑21 (MQ=255)                                       aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacat    <  1:85290‑M1/49‑24 (MQ=255)                                       aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca     <  1:2269864‑M1/48‑23 (MQ=255)                                         ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat  >  1:1507444‑M1/1‑24 (MQ=255)                                               |                                            TTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACA  >  NC_000913/765143‑765232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑