Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG63.6% 4.7 / 11.3 11V288G (GTG→GGG) cusAcopper/silver export system RND permease
Reads supporting (aligned to +/- strand):  ref base T (0/4);  new base G (7/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 3.03e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCC  >  NC_000913/599531‑599622
                                             |                                              
ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGggggggg                                             >  1:380019/1‑48 (MQ=255)
     aTTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATcc                                        >  1:1939209/1‑49 (MQ=38)
     aTTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATcc                                        >  1:2718705/1‑49 (MQ=39)
          cGAACTAAGCGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGa                                   >  1:567349/1‑49 (MQ=38)
                 aaCGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGc                             <  1:539730/48‑1 (MQ=255)
                    ggcgaaggcgaaGTGGCGGGGGGGGGGGGGATCCTGCGATCCGGCaaa                          >  1:632147/1‑48 (MQ=38)
                     gcgaaggcgaagTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCaaaa                         >  1:2668904/1‑48 (MQ=255)
                      cgaaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAAc                       <  1:551061/49‑1 (MQ=255)
                      cgaaggcgaagTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAAc                       >  1:1721441/1‑49 (MQ=255)
                         aggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGcc                    <  1:360831/49‑1 (MQ=255)
                             gaagTGGCGGGCGGGGGGGGGGTCCTGCGGTCCGGCAAAAAAACCCgg                 >  1:2051578/1‑47 (MQ=19)
                                         ggggTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcgccg    <  1:1264945/49‑1 (MQ=255)
                                           ggtggtGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCgccgcc  <  1:1085710/49‑1 (MQ=255)
                                           ggtggtGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCgccgcc  <  1:2797311/49‑1 (MQ=255)
                                             |                                              
GGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCC  >  NC_000913/599531‑599622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑