Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,086	C→T	intergenic (‑300/‑77)	yaaI ← / → dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,086	0	C	T	100.0%	122.6 / NA	32	intergenic (‑300/‑77)	yaaI/dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/15);  total (17/15)

GTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCAC  >  NC_000913/12042‑12130                                             |                                             gtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGt                                           <  1:995422/48‑1 (MQ=255)  tagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTg                                         <  1:750807/49‑1 (MQ=255)     tCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGaaa                                      <  1:2858820/49‑1 (MQ=255)       aaCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAcc                                    <  1:85450/49‑1 (MQ=255)       aaCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAcc                                    <  1:261547/49‑1 (MQ=255)        aCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                     >  1:2379431/1‑47 (MQ=255)        aCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                     >  1:55750/1‑47 (MQ=255)             agtgagtgAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGt                              <  1:1762189/49‑1 (MQ=255)              gtgagtgaGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGtt                             >  1:1896539/1‑49 (MQ=255)                gagtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTc                           >  1:850472/1‑49 (MQ=255)                gagtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTc                           >  1:517613/1‑49 (MQ=255)                 agtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTc                           <  1:934032/48‑1 (MQ=255)                 agtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTc                           <  1:1377561/48‑1 (MQ=255)                 agtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCg                          >  1:2146569/1‑49 (MQ=255)                   tgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGcc                        >  1:2166946/1‑49 (MQ=255)                   tgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGcc                        >  1:1449617/1‑49 (MQ=255)                    gagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGccc                       >  1:508956/1‑49 (MQ=255)                     agtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGcccc                      >  1:1100150/1‑49 (MQ=255)                     agtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGcccc                      <  1:342873/49‑1 (MQ=255)                       tCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTa                    >  1:2760309/1‑49 (MQ=255)                       tCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTa                    <  1:944332/49‑1 (MQ=255)                        cTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTAt                   >  1:2268162/1‑49 (MQ=255)                        cTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTAt                   >  1:1798832/1‑49 (MQ=255)                          gCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa                 >  1:2460134/1‑49 (MQ=255)                            aaaaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACa               <  1:2581534/49‑1 (MQ=255)                                 aaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTc          <  1:1225849/49‑1 (MQ=255)                                  aTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTca         >  1:454784/1‑49 (MQ=255)                                  aTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTca         <  1:2756999/49‑1 (MQ=255)                                   tGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTca         >  1:305187/1‑48 (MQ=255)                                    gAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTcac        >  1:869255/1‑48 (MQ=255)                                       aTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAAcc    <  1:293005/49‑1 (MQ=255)                                          gggTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACcac  <  1:1477505/48‑1 (MQ=255)                                             |                                             GTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCAC  >  NC_000913/12042‑12130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑