Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,093,223	A→G	V72V (GTT→GTC)	rhaM ←	L‑rhamnose mutarotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,093,223	0	A	G	100.0%	201.8 / NA	52	V72V (GTT→GTC)	rhaM	L‑rhamnose mutarotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/29);  total (23/29)

GGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATC  >  NC_000913/4093177‑4093268                                               |                                              ggTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGAc                                             <  1:91006/49‑1 (MQ=255)   ttAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt                                           <  1:2387550/49‑1 (MQ=255)     aGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATc                                          <  1:1398342/48‑1 (MQ=255)      gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCa                                         <  1:163973/48‑1 (MQ=255)      gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCa                                         <  1:2610342/48‑1 (MQ=255)      gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCa                                         <  1:1471183/48‑1 (MQ=255)       cGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGt                                       <  1:2558724/49‑1 (MQ=255)       cGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGt                                       <  1:766984/49‑1 (MQ=255)        gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg                                      <  1:1666507/49‑1 (MQ=255)         ggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGc                                     >  1:633140/1‑49 (MQ=255)         ggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGc                                     <  1:2623544/49‑1 (MQ=255)          gCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCt                                    >  1:2845751/1‑49 (MQ=255)          gCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCt                                    <  1:1876742/49‑1 (MQ=255)           cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                   <  1:2846127/49‑1 (MQ=255)           cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                   <  1:2518420/49‑1 (MQ=255)           cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                   <  1:1183469/49‑1 (MQ=255)             tAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg                                  >  1:665463/1‑48 (MQ=255)             tAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc                                 <  1:1808533/49‑1 (MQ=255)              aaCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc                                 >  1:2356238/1‑48 (MQ=255)              aaCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc                                 >  1:2132370/1‑48 (MQ=255)               aCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCa                                <  1:5578/48‑1 (MQ=255)                  tCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                            >  1:1501984/1‑49 (MQ=255)                  tCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                            >  1:1333378/1‑49 (MQ=255)                  tCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                            >  1:939249/1‑49 (MQ=255)                    ggTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCa                          >  1:2610114/1‑49 (MQ=255)                    ggTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCa                          <  1:969635/49‑1 (MQ=255)                    ggTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCa                          >  1:1746499/1‑49 (MQ=255)                     gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt                         <  1:1960256/49‑1 (MQ=255)                     gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt                         <  1:876815/49‑1 (MQ=255)                     gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt                         >  1:2764310/1‑49 (MQ=255)                        atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTcc                      <  1:2698602/49‑1 (MQ=255)                        atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                       >  1:2766857/1‑48 (MQ=255)                         tatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCa                     <  1:789653/49‑1 (MQ=255)                          atatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAg                    >  1:1528408/1‑49 (MQ=255)                           tatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGc                   >  1:1593963/1‑49 (MQ=255)                            atTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCg                  >  1:1558422/1‑49 (MQ=255)                               tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc               >  1:1167187/1‑49 (MQ=255)                               tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGtt                <  1:10842/48‑1 (MQ=255)                                ccaccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc               <  1:1113456/48‑1 (MQ=255)                                ccaccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc               <  1:2537366/48‑1 (MQ=255)                                 caccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt             <  1:437467/49‑1 (MQ=255)                                  accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttcttc            <  1:1480963/49‑1 (MQ=255)                                  accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt             >  1:1195591/1‑48 (MQ=255)                                    caACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt             >  1:1827425/1‑46 (MQ=255)                                    caACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt             >  1:1441363/1‑46 (MQ=255)                                    caACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAg          <  1:2754636/49‑1 (MQ=255)                                       cGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt       >  1:1487088/1‑49 (MQ=255)                                       cGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt       <  1:1517631/49‑1 (MQ=255)                                        gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt       >  1:1606169/1‑48 (MQ=255)                                         ttGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc      >  1:1395407/1‑48 (MQ=255)                                         ttGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc      >  1:399115/1‑48 (MQ=255)                                            gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtc  <  1:960614/49‑1 (MQ=255)                                               |                                              GGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATC  >  NC_000913/4093177‑4093268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑