Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,124	T→C	W140R (TGG→CGG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,124	0	T	C	100.0%	134.8 / NA	35	W140R (TGG→CGG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/21);  total (14/21)

AAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATT  >  NC_000913/4438079‑4438168                                              |                                             aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                            >  1:705344/1‑48 (MQ=255)  aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                          <  1:1506259/49‑1 (MQ=255)   aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                         <  1:1060888/49‑1 (MQ=255)   aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                         <  1:2821299/49‑1 (MQ=255)   aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                          >  1:2265876/1‑48 (MQ=255)      ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                      <  1:1996162/49‑1 (MQ=255)      ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                      >  1:2602565/1‑49 (MQ=255)        gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                    <  1:805954/49‑1 (MQ=255)           aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                   >  1:1757604/1‑47 (MQ=255)            aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                <  1:1325408/49‑1 (MQ=255)             tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                >  1:297845/1‑48 (MQ=255)                ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaa                             <  1:543820/48‑1 (MQ=255)                ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaa                             <  1:1859976/48‑1 (MQ=255)                 cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                            <  1:763043/48‑1 (MQ=255)                   ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                          >  1:289243/1‑48 (MQ=255)                   ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                          >  1:2393218/1‑48 (MQ=255)                      gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                      <  1:463323/49‑1 (MQ=255)                       aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                     >  1:2031708/1‑49 (MQ=255)                        aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                    <  1:2648736/49‑1 (MQ=255)                         cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                    >  1:2449411/1‑48 (MQ=255)                           aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                  >  1:1519042/1‑48 (MQ=255)                           aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGAc                 <  1:2539479/49‑1 (MQ=255)                             aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                >  1:2125956/1‑48 (MQ=255)                               aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc             <  1:3908/49‑1 (MQ=255)                                 aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg           <  1:1061939/49‑1 (MQ=255)                                    aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa        <  1:349826/49‑1 (MQ=255)                                    aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa        <  1:1810228/49‑1 (MQ=255)                                     ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACTGaa         >  1:2508405/1‑47 (MQ=39)                                     ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa       <  1:206498/49‑1 (MQ=255)                                       gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg      >  1:2590607/1‑48 (MQ=255)                                       gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg      <  1:1443403/48‑1 (MQ=255)                                         aTGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt   >  1:149056/1‑49 (MQ=255)                                          tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt  <  1:451463/49‑1 (MQ=255)                                          tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt  <  1:1129105/49‑1 (MQ=255)                                          tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt  <  1:915765/49‑1 (MQ=255)                                              |                                             AAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATT  >  NC_000913/4438079‑4438168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑