Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	76.8 / NA	20	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/8);  total (12/8)

GAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAG  >  NC_000913/988455‑988544                                             |                                              gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                           >  1:1093473/1‑49 (MQ=255)     aTTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaaa                                       <  1:1476375/49‑1 (MQ=255)        tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                     >  1:375083/1‑48 (MQ=255)               cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAgaagaa                             <  1:187561/49‑1 (MQ=255)                aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                            <  1:626469/49‑1 (MQ=255)                  cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATcc                          <  1:583112/49‑1 (MQ=255)                  cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATcc                          >  1:550476/1‑49 (MQ=255)                   ttCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                         >  1:14309/1‑49 (MQ=255)                    tCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                        >  1:1099346/1‑49 (MQ=255)                      ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                      <  1:2693474/49‑1 (MQ=255)                       gTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                     <  1:803524/49‑1 (MQ=255)                        tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc                    >  1:2748355/1‑49 (MQ=255)                         aTCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                   >  1:2746186/1‑49 (MQ=255)                          tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                  >  1:1085763/1‑49 (MQ=255)                           cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                  >  1:2441180/1‑48 (MQ=255)                            aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                 >  1:2006597/1‑48 (MQ=255)                                 cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag             >  1:2027710/1‑47 (MQ=255)                                  ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaag          >  1:149299/1‑49 (MQ=255)                                   gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc         <  1:1797661/49‑1 (MQ=255)                                          gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg  <  1:2638208/49‑1 (MQ=255)                                             |                                              GAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAG  >  NC_000913/988455‑988544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑